Impact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men.
Impact of precise modulation of reactive oxygen species levels on spermatozoa proteins in infertile men.
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活性氧水平的精确调节对不育男性的精子蛋白的影响。
DOI：
10.1186/1559-0275-12-4
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发表时间：
2015
影响因子：
3.8
通讯作者：
Cui Z
中科院分区：
文献类型：
--
作者：
Ayaz A;Agarwal A;Sharma R;Arafa M;Elbardisi H;Cui Z
在25%至80%的不育男性中检测到活性氧（ROS）水平升高。它们与男性不育的病理有关。了解活性氧水平升高对精子蛋白差异表达的影响，对于了解可能涉及男性不育的细胞过程和/ /途径非常重要。本研究的目的是检测低、中、高ROS水平患者精子中的差异表达蛋白（DEPs）。共有42名不育症男性和17名证明有生育能力的男性参加了这项研究。化学发光法测定ROS水平。不孕症男性分为低（0 ~ < 93 RLU/s/106精子）组（n = 11）、中（93 ~ 500 RLU/s/106精子）组（n = 17）和高（500 RLU/s/106精子）组（n = 14）。所有有生育能力的男性的ROS水平在4-50 RLU/s/106精子之间。可育组4例，低、中、高ROS组各4例。进行了蛋白质提取、蛋白质鉴定、凝胶分离、凝胶内消化、LTQ-orbitrap精英混合质谱分析系统。利用生物信息学工具研究了DEPs及其细胞定位和通路。通过整体蛋白质组学分析，在3组中共鉴定出1035个蛋白。其中，305人是dep。低ROS组51例，中ROS组47例，高ROS组104例。通过Uniprot和DAVID鉴定出6个具有明显生殖功能的DEPs，它们仅在3个ROS组中表达，而在对照组中不表达。我们首次证实了6种具有不同生殖功能的dep仅存在于ROS水平低、中或高的男性中。这些dep可以作为氧化应激诱导的男性不育的潜在生物标志物。本文的在线版本（doi:10.1186/1559-0275-12-4）包含补充材料，可供授权用户使用。
Elevated levels of reactive oxygen species (ROS) are detected in 25% to 80% of infertile men. They are involved in the pathology of male infertility. Understanding the effect of increasing levels of ROS on the differential expression of sperm proteins is important to understand the cellular processes and or/pathways that may be implicated in male infertility. The aim of this study was to examine differentially expressed proteins (DEPs) in spermatozoa from patients with low, medium and high ROS levels. A total of 42 infertile men presenting for infertility and 17 proven fertile men were enrolled in the study. ROS levels were measured by chemiluminescence assay. Infertile men were divided into Low (0- < 93 RLU/s/106 sperm) (n = 11), Medium (>93-500 RLU/s/106 sperm) (n = 17) and High ROS (>500 RLU/s/106 sperm) group (n = 14). All fertile men had ROS levels between 4-50 RLU/s/106 sperm. 4 subjects from fertile group and 4 each from the Low, Medium and High ROS were pooled. Protein extraction, protein estimation, gel separation of the proteins, in-gel digestion, LTQ-orbitrap elite hybrid mass spectrometry system was conducted. The DEPs, the cellular localization and pathways of DEPs involved were examined utilizing bioinformatics tools. 1035 proteins were identified in the 3 groups by global proteomic analysis. Of these, 305 were DEPs. 51 were unique to the Low ROS group, 47 Medium ROS group and 104 were unique to the High ROS group. 6 DEPs were identified by Uniprot and DAVID that had distinct reproductive functions and they were expressed only in 3 ROS groups but not in the control. We have for the first time demonstrated the presence of 6 DEPs with distinct reproductive functions only in men with low, medium or high ROS levels. These DEPs can serve as potential biomarkers of oxidative stress induced male infertility. The online version of this article (doi:10.1186/1559-0275-12-4) contains supplementary material, which is available to authorized users.
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影响因子：
7.4
作者：
Bhatia, Vivek N.;Perlman, David H.;Costello, Catherine E.;McComb, Mark E.
通讯作者：
McComb, Mark E.
DOI：
10.1007/s13361-011-0237-2
发表时间：
2011-12-01
影响因子：
3.2
作者：
Gokce, Emine;Shuford, Christopher M.;Muddiman, David C.
通讯作者：
Muddiman, David C.
影响因子：
9.8
作者：
Loges, Niki Tomas;Olbrich, Heike;Omran, Heymut
通讯作者：
Omran, Heymut
影响因子：
3.4
作者：
Martinez-Heredia, Juan;Estanyol, Josep Maria;Oliva, Rafael
通讯作者：
Oliva, Rafael
DOI：
10.5534/wjmh.2014.32.1.1
发表时间：
2014-04
期刊：
The world journal of men's health
影响因子：
--
作者：
Agarwal A;Virk G;Ong C;du Plessis SS
通讯作者：
du Plessis SS