Tumor vasculature targeting and imaging in living mice with reduced graphene oxide.
Tumor vasculature targeting and imaging in living mice with reduced graphene oxide.
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DOI：
10.1016/j.biomaterials.2013.01.047
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发表时间：
2013-04
期刊：
影响因子：
14
通讯作者：
Cai, Weibo
中科院分区：
文献类型：
--
作者：
Shi, Sixiang;Yang, Kai;Hong, Hao;Valdovinos, Hector F.;Nayak, Tapas R.;Zhang, Yin;Theuer, Charles P.;Barnhart, Todd E.;Liu, Zhuang;Cai, Weibo
关键词：
石墨烯基纳米材料因其独特的性质在生物医学领域引起了广泛的关注。在此，我们报告了使用与抗CD 105抗体TRC 105缀合的还原氧化石墨烯（RGO）在活小鼠中的肿瘤血管系统靶向和成像。RGO偶联物64 Cu-NOTA-RGO-TRC 105在体外和体内表现出优异的稳定性。系列正电子发射断层扫描（PET）成像研究非侵入性地评估了药代动力学，并证明了与非靶向RGO缀合物（64 Cu-NOTA-RGO）相比，64 Cu-NOTA-RGO-TRC 105在体内特异性靶向4 T1鼠乳腺肿瘤。体内（例如，用过量的TRC 105阻断4 T1肿瘤摄取），体外（例如，流式细胞术），和离体（例如，组织学）实验证实了64 Cu-NOTA-RG 0-TRC 105对肿瘤血管CD 105的特异性。由于RGO具有光热治疗所需的特性，在这项工作中开发的肿瘤特异性RGO缀合物可以作为一个有前途的治疗诊断剂，整合成像和治疗成分。
Graphene-based nanomaterials have attracted tremendous attention in the field of biomedicine due to their intriguing properties. Herein, we report tumor vasculature targeting and imaging in living mice using reduced graphene oxide (RGO), which was conjugated to the anti-CD105 antibody TRC105. The RGO conjugate, 64Cu-NOTA-RGO-TRC105, exhibited excellent stability in vitro and in vivo. Serial positron emission tomography (PET) imaging studies non-invasively assessed the pharmacokinetics and demonstrated specific targeting of 64Cu-NOTA-RGO-TRC105 to 4T1 murine breast tumors in vivo, compared to non-targeted RGO conjugate (64Cu-NOTA-RGO). In vivo (e.g., blocking 4T1 tumor uptake with excess TRC105), in vitro (e.g., flow cytometry), and ex vivo (e.g., histology) experiments confirmed the specificity of 64Cu-NOTA-RGO-TRC105 for tumor vascular CD105. Since RGO exhibits desirable properties for photothermal therapy, the tumor-specific RGO conjugate developed in this work may serve as a promising theranostic agent that integrates imaging and therapeutic components.
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影响因子：
3.1
作者：
Dearling JL;Voss SD;Dunning P;Snay E;Fahey F;Smith SV;Huston JS;Meares CF;Treves ST;Packard AB
通讯作者：
Packard AB
石墨烯在生物医学中的机遇与挑战
影响因子：
5.5
作者：
Feng, Liangzhu;Liu, Zhuang
通讯作者：
Liu, Zhuang
影响因子：
17.1
作者：
Hong H;Yang K;Zhang Y;Engle JW;Feng L;Yang Y;Nayak TR;Goel S;Bean J;Theuer CP;Barnhart TE;Liu Z;Cai W
通讯作者：
Cai W
影响因子：
41.2
作者：
Geim, A. K.;Novoselov, K. S.
通讯作者：
Novoselov, K. S.
影响因子：
14.8
作者：
Cai, Weibo;Chen, Xiaoyuan
通讯作者：
Chen, Xiaoyuan