In vivo targeting and imaging of tumor vasculature with radiolabeled, antibody-conjugated nanographene.
In vivo targeting and imaging of tumor vasculature with radiolabeled, antibody-conjugated nanographene.
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DOI：
10.1021/nn204625e
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发表时间：
2012-03-27
期刊：
影响因子：
17.1
通讯作者：
Cai W
中科院分区：
文献类型：
--
作者：
Hong H;Yang K;Zhang Y;Engle JW;Feng L;Yang Y;Nayak TR;Goel S;Bean J;Theuer CP;Barnhart TE;Liu Z;Cai W
在这里，我们证明了纳米石墨烯可以通过靶向肿瘤血管生成的血管标记物CD105(即endoglin)在体内特异性地靶向肿瘤新生血管。共价官能化的纳米氧化石墨烯(GO)具有良好的稳定性和靶向性。通过一系列无创正电子发射断层扫描(PET)成像和生物分布研究，研究了GO结合物的药代动力学和肿瘤靶向性，并通过体外、体内和体外实验进行了验证。主动靶向配体(TRC105，一种与CD105结合的单抗)的加入显著提高了肿瘤对功能化GO的摄取，这种功能是针对新生血管的，几乎没有渗出，因此有必要进一步研究这些GO结合物用于癌症靶向药物输送和/或光热治疗，以提高治疗效果。由于渗出不良是基于纳米材料的肿瘤体内靶向的主要障碍，本研究也将CD105确立为未来癌症纳米医学的有希望的血管靶点。
Herein we demonstrate that nano-graphene can be specifically directed to the tumor neovasculature in vivo through targeting of CD105 (i.e. endoglin), a vascular marker for tumor angiogenesis. The covalently functionalized nano-graphene oxide (GO) exhibited excellent stability and target specificity. Pharmacokinetics and tumor targeting efficacy of the GO conjugates were investigated with serial non-invasive positron emission tomography (PET) imaging and biodistribution studies, which were validated by in vitro, in vivo, and ex vivo experiments. The incorporation of an active targeting ligand (TRC105, a monoclonal antibody that binds to CD105) led to significantly improved tumor uptake of functionalized GO, which was specific for the neovasculature with little extravasation, warranting future investigation of these GO conjugates for cancer-targeted drug delivery and/or photothermal therapy to enhance therapeutic efficacy. Since poor extravasation is a major hurdle for nanomaterial-based tumor targeting in vivo, this study also establishes CD105 as a promising vascular target for future cancer nanomedicine.
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影响因子：
10.8
作者：
Hong H;Shi J;Yang Y;Zhang Y;Engle JW;Nickles RJ;Wang X;Cai W
通讯作者：
Cai W
影响因子：
3.1
作者：
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影响因子：
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作者：
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影响因子：
15
作者：
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影响因子：
13.3
作者：
Bao, Hongqian;Pan, Yongzheng;Gan, Leong Huat
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