The Chlamydomonas cell wall and its constituent glycoproteins analyzed by the quick-freeze, deep-etch technique.
The Chlamydomonas cell wall and its constituent glycoproteins analyzed by the quick-freeze, deep-etch technique.
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DOI：
10.1083/jcb.101.4.1550
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发表时间：
1985-10
期刊：
影响因子：
--
通讯作者：
Heuser JE
中科院分区：
文献类型：
--
作者：
Goodenough UW;Heuser JE
使用快速冷冻，深蚀刻技术，我们分析了完整的细胞壁的衣原体reinhardi的结构，并可视化其组成糖蛋白后，机械剪切和解聚诱导高氯酸盐或壁破坏剂，自溶素。完整的壁先前已经在薄切片研究中显示（Roberts，K.，M. Gurney-Smith和G. J. Hills，1972，J. Ultrastruct. Res. 40：599-613）以由将原纤维的网状物平分的离散的中心三联体组成。深蚀刻技术提供了额外的信息，这些层中的每一个在几个不同的实验条件下的架构，并证明，每一层是由一组不同的组件。中心三联体的最内层被证明是一个纤维状网络，它对高氯酸盐稳定，但被自溶素破坏，分解成纤维状单元，我们称之为“鱼骨”。“三联体的中间层是一个松散的大颗粒组合。外层是一个薄的，结晶组装，是相对不受自溶。它解聚成两种糖蛋白，一种是纤维状的，一种是球状的。壁糖蛋白被证明在结构上类似于与鞭毛膜相关的两种纤维蛋白，即性凝集素和我们称为“吊床”的结构的原体。“它们也与多细胞植物和动物的细胞外基质中发现的一些纤维成分同源。快速冷冻，深蚀刻技术被证明是一种高度信息化的方式来解剖纤维基质的结构和可视化其组成大分子。
Using the quick-freeze, deep-etch technique, we have analyzed the structure of the intact cell wall of Chlamydomonas reinhardi, and have visualized its component glycoproteins after mechanical shearing and after depolymerization induced by perchlorate or by the wall-disrupting agent, autolysin. The intact wall has previously been shown in a thin- section study (Roberts, K., M. Gurney-Smith, and G. J. Hills, 1972, J. Ultrastruct. Res. 40:599-613) to consist of a discrete central triplet bisecting a meshwork of fibrils. The deep-etch technique provides additional information about the architecture of each of these layers under several different experimental conditions, and demonstrates that each layer is constructed from a distinct set of components. The innermost layer of the central triplet proves to be a fibrous network which is stable to perchlorate but destabilized by autolysin, disassembling into fibrillar units we designate as "fishbones." The medial layer of the triplet is a loose assemblage of large granules. The outer layer is a thin, crystalline assembly that is relatively unaffected by autolysin. It depolymerizes into two glycoprotein species, one fibrous and one globular. The wall glycoproteins prove to be structurally similar to two fibrous proteins that associate with the flagellar membrane, namely, the sexual agglutinins and the protomers of a structure we designate a "hammock." They are also homologous to some of the fibrous components found in the extracellular matrices of multicellular plants and animals. The quick-freeze, deep-etch technique is demonstrated to be a highly informative way to dissect the structure of a fibrous matrix and visualize its component macromolecules.
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DOI：
10.1083/jcb.84.3.560
发表时间：
1980-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heuser J
通讯作者：
Heuser J
影响因子：
5.6
作者：
HEUSER, JE
通讯作者：
HEUSER, JE
影响因子：
5.6
作者：
HILLS, GJ;PHILLIPS, JM;ROBERTS, K
通讯作者：
ROBERTS, K
DOI：
10.1017/s0016672300012015
发表时间：
1971-01-01
期刊：
GENETICAL RESEARCH
影响因子：
--
作者：
DAVIES, DR;PLASKITT, A
通讯作者：
PLASKITT, A
DOI：
10.1007/bf00424874
发表时间：
1971-01-01
期刊：
ARCHIV FUR MIKROBIOLOGIE
影响因子：
--
作者：
CLAES, H
通讯作者：
CLAES, H