Differential Gene Expression in the Heads of Behaviorally Divergent Culex pipiens Mosquitoes.
Differential Gene Expression in the Heads of Behaviorally Divergent Culex pipiens Mosquitoes.
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行为分化的尖音库蚊头部的差异基因表达
DOI：
10.3390/insects12030271
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发表时间：
2021-03-23
期刊：
影响因子：
3
通讯作者：
Fritz ML
中科院分区：
文献类型：
--
作者：
Noreuil A;Fritz ML
宿主偏好对蚊媒疾病传播的流行病学有深远影响，但这些偏好的分子基础仍有许多未知之处。在这里，我们研究了西尼罗河病毒媒介库蚊的宿主偏好。我们研究了8个种群的人类和鸟类着陆率：5个种群来自地上，3个种群来自地下繁殖和越冬栖息地。虽然地上种群倾向于鸟类着陆，地下种群倾向于人类着陆，但观察到了一系列行为，与其他蚊子物种一致。作为识别参与调节取血行为的基因以及宿主偏好差异的第一步，我们测量了一个鸟类和一个人类寻找群体的差异表达模式和剪接位点变异。最后，我们对特定的差异表达基因及其影响寄主寻找行为的可能性进行了讨论。雌性比目鱼。CX的主机首选项。蚊子是西尼罗河病毒传播给人类的桥梁载体，有可能推动病原体的传播动力学。然而，关于这些偏好的差异程度及其分子基础，仍有许多未知之处。我们在实验室环境中进行了宿主选择分析，以量化CX的多天人类和鸟类着陆率。雌性比目鱼。被测种群来自5个地上和3个地下繁殖和越冬栖息地。所有三个地下种群都偏向于人类着陆，人类登陆率从69%到85%不等。在五个地上种群中，四个种群的鸟类着陆率为80%，而一个种群仅有44%的时间降落在鸟类宿主上。在多天的测试中，总体应答率和交替降落在人类和禽类宿主上的意愿也因种群而异。对于一个偏爱人类和一个偏爱鸟类的种群，我们研究了在雌性头部表达的基因的差异表达和剪接位置变异的模式。我们还比较了在怀孕或寻找寄主的生理状态下寻找人类的雌性动物的基因表达和剪接位置的变化，以确定可能调节血液摄食行为的基因。总体而言，我们确定了具有代谢和调节功能的基因，这些基因在我们对怀孕和寻找宿主的女性的比较中有差异表达。在我们对寻找鸟类和人类的雌性动物的比较中，差异表达的基因在那些参与感官感知的女性中得到了丰富。最后，我们讨论了特定的感觉基因及其对寻找鸟类和人类的CX的不同行为的潜在影响。皮皮恩斯。
Host preference has profound impacts on the epidemiology of mosquito-borne disease transmission, yet much is still unknown about the molecular basis for these preferences. Here, we examined host preference in the West Nile virus vector, Culex pipiens. We examined human and avian landing rates for eight populations: five originating from above- and three from below-ground breeding and overwintering habitats. While above-ground populations tended to be biased toward avian landing and below-ground populations tended toward human landing, a range of behaviors was observed, consistent with other mosquito species. Patterns of differential expression and splice site variation were measured for one avian- and one human-seeking population as a first step toward identifying genes involved in regulation of blood feeding behaviors as well as differences in host preference. We conclude with a discussion of specific differentially expressed genes and their potential to influence host seeking behaviors of Cx. pipiens females. Host preferences of Cx. pipiens, a bridge vector for West Nile virus to humans, have the potential to drive pathogen transmission dynamics. Yet much remains unknown about the extent of variation in these preferences and their molecular basis. We conducted host choice assays in a laboratory setting to quantify multi-day human and avian landing rates for Cx. pipiens females. Assayed populations originated from five above-ground and three below-ground breeding and overwintering habitats. All three below-ground populations were biased toward human landings, with rates of human landing ranging from 69–85%. Of the five above-ground populations, four had avian landing rates of >80%, while one landed on the avian host only 44% of the time. Overall response rates and willingness to alternate landing on the human and avian hosts across multiple days of testing also varied by population. For one human- and one avian-preferring population, we examined patterns of differential expression and splice site variation at genes expressed in female heads. We also compared gene expression and splice site variation within human-seeking females in either gravid or host-seeking physiological states to identify genes that may regulate blood feeding behaviors. Overall, we identified genes with metabolic and regulatory function that were differentially expressed in our comparison of gravid and host-seeking females. Differentially expressed genes in our comparison of avian- and human-seeking females were enriched for those involved in sensory perception. We conclude with a discussion of specific sensory genes and their potential influence on the divergent behaviors of avian- and human-seeking Cx. pipiens.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.7
作者：
Freeman EG;Dahanukar A
通讯作者：
Dahanukar A
卵巢的雌激素激素独立于黄热病蚊子中的胰岛素受体，埃及埃及。
影响因子：
3.8
作者：
Dhara A;Eum JH;Robertson A;Gulia-Nuss M;Vogel KJ;Clark KD;Graf R;Brown MR;Strand MR
通讯作者：
Strand MR
影响因子：
--
作者：
Fritz ML;Walker ED;Yunker AJ;Dworkin I
通讯作者：
Dworkin I
影响因子：
56.9
作者：
Fonseca, DM;Keyghobadi, N;Wilkerson, RC
通讯作者：
Wilkerson, RC