The physiology and proteomics of drought tolerance in maize: early stomatal closure as a cause of lower tolerance to short-term dehydration?
The physiology and proteomics of drought tolerance in maize: early stomatal closure as a cause of lower tolerance to short-term dehydration?
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DOI：
10.1371/journal.pone.0038017
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hniličková H
中科院分区：
文献类型：
--
作者：
Benešová M;Holá D;Fischer L;Jedelský PL;Hnilička F;Wilhelmová N;Rothová O;Kočová M;Procházková D;Honnerová J;Fridrichová L;Hniličková H
了解作物对干旱的反应是培育耐受基因型的第一步。在本研究中，两个玉米（Zea mays L.）对脱水敏感性不同的基因型经受中度干旱条件。随后的生理参数的分析表明，气孔导度下降伴随着轻微的相对含水量的减少在敏感的基因型。与此相反，耐受基因型保持开放的气孔和活跃的光合作用，即使在脱水条件下。干旱诱导的变化在叶蛋白质组进行了分析，两个独立的方法，2D凝胶电泳和iTRAQ分析，这提供了兼容的，但只有部分重叠的结果。干旱导致两种基因型的保护和应激相关蛋白（主要是分子伴侣和蛋白酶）的上调。各种解毒蛋白水平的差异与所观察到的抗氧化酶活性的变化相对应。上调的保护蛋白的数量和水平一般较低的敏感基因型，这意味着蛋白合成水平降低，这也表明了特定的变化的翻译机器的组件。基于这些结果，我们提出，在敏感的基因型中的超敏感的早期气孔关闭导致光合作用的抑制，随后，与耐旱性相关的保护/解毒蛋白质的合成效率较低。
Understanding the response of a crop to drought is the first step in the breeding of tolerant genotypes. In our study, two maize (Zea mays L.) genotypes with contrasting sensitivity to dehydration were subjected to moderate drought conditions. The subsequent analysis of their physiological parameters revealed a decreased stomatal conductance accompanied by a slighter decrease in the relative water content in the sensitive genotype. In contrast, the tolerant genotype maintained open stomata and active photosynthesis, even under dehydration conditions. Drought-induced changes in the leaf proteome were analyzed by two independent approaches, 2D gel electrophoresis and iTRAQ analysis, which provided compatible but only partially overlapping results. Drought caused the up-regulation of protective and stress-related proteins (mainly chaperones and dehydrins) in both genotypes. The differences in the levels of various detoxification proteins corresponded well with the observed changes in the activities of antioxidant enzymes. The number and levels of up-regulated protective proteins were generally lower in the sensitive genotype, implying a reduced level of proteosynthesis, which was also indicated by specific changes in the components of the translation machinery. Based on these results, we propose that the hypersensitive early stomatal closure in the sensitive genotype leads to the inhibition of photosynthesis and, subsequently, to a less efficient synthesis of the protective/detoxification proteins that are associated with drought tolerance.
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
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