Molecular cloning and expression of CYP9A61: a chlorpyrifos-ethyl and lambda-cyhalothrin-inducible cytochrome P450 cDNA from Cydia pomonella.
Molecular cloning and expression of CYP9A61: a chlorpyrifos-ethyl and lambda-cyhalothrin-inducible cytochrome P450 cDNA from Cydia pomonella.
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DOI：
10.3390/ijms141224211
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发表时间：
2013-12-13
影响因子：
5.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Yang X;Li X;Zhang Y
细胞色素 P450 单加氧酶（CYP 或 P450）在许多害虫的杀虫剂解毒中发挥着至关重要的作用。然而，人们对 P450 的作用及其对苹果蠹蛾 Cydia pomonella (L.)（一种经济上重要的水果害虫）中杀虫剂暴露的反应知之甚少。在此，我们报告了该害虫的第一个 P450 基因（命名为 CYP9A61）的特征和表达分析。 CYP9A61的cDNA全长序列长2071bp，其开放阅读框(ORF)编码538个氨基酸。序列分析显示，CYP9A61 与其他已知 CYP9 具有 51%–60% 的同一性，并包含高度保守的底物识别位点 SRS1、SRS4 和 SRS5。实时定量PCR显示，CYP9A61在五龄幼虫中的表达量是一龄幼虫的67倍，并且在丝腺和脂肪体中的含量比其他组织更丰富。将 3 龄幼虫暴露于 12.5 mg L−1 乙基毒死蜱 60 小时和 0.19 mg L−1 高效氯氟氰菊酯 36 小时，分别导致 CYP9A61 诱导 2.20 倍和 3.47 倍。 3龄幼虫接触这两种杀虫剂也显着增强了总P450活性。结果表明CYP9A61是一种杀虫解毒P450。
Cytochrome P450 monooxygenases (CYPs or P450s) play paramount roles in detoxification of insecticides in a number of insect pests. However, little is known about the roles of P450s and their responses to insecticide exposure in the codling moth Cydia pomonella (L.), an economically important fruit pest. Here we report the characterization and expression analysis of the first P450 gene, designated as CYP9A61, from this pest. The full-length cDNA sequence of CYP9A61 is 2071 bp long and its open reading frame (ORF) encodes 538 amino acids. Sequence analysis shows that CYP9A61 shares 51%–60% identity with other known CYP9s and contains the highly conserved substrate recognition site SRS1, SRS4 and SRS5. Quantitative real-time PCR showed that CYP9A61 were 67-fold higher in the fifth instar larvae than in the first instar, and more abundant in the silk gland and fat body than other tissues. Exposure of the 3rd instar larvae to 12.5 mg L−1 of chlorpyrifos-ethyl for 60 h and 0.19 mg L−1 of lambda-cyhalothrin for 36 h resulted in 2.20-and 3.47-fold induction of CYP9A61, respectively. Exposure of the 3rd instar larvae to these two insecticides also significantly enhanced the total P450 activity. The results suggested that CYP9A61 is an insecticide-detoxifying P450.
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影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
23.8
作者：
Arrese EL;Soulages JL
通讯作者：
Soulages JL
影响因子：
2.2
作者：
Bouvier, JC;Boivin, T;Sauphanor, B
通讯作者：
Sauphanor, B
DOI：
10.1073/pnas.0812141106
发表时间：
2009-04-07
影响因子：
11.1
作者：
Chung, Henry;Sztal, Tamar;Daborn, Phillip J.
通讯作者：
Daborn, Phillip J.
影响因子：
3.8
作者：
Komagata, Osamu;Kasai, Shinji;Tomita, Takashi
通讯作者：
Tomita, Takashi