Nanoparticles for improved local retention after intra-articular injection into the knee joint.
Nanoparticles for improved local retention after intra-articular injection into the knee joint.
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DOI：
10.1007/s11095-012-0870-x
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发表时间：
2013-01
影响因子：
3.7
通讯作者：
Tortorella, Micky
中科院分区：
文献类型：
--
作者：
Morgen, Michael;Tung, David;Boras, Britton;Miller, Warren;Malfait, Anne-Marie;Tortorella, Micky
评价使用与透明质酸盐相互作用形成离子交联水凝胶的阳离子聚合物纳米颗粒增加骨关节炎药物在滑膜腔中的关节内滞留时间。 体外试验包括使用注射器和膜溶解试验从交联水凝胶中释放纳米颗粒，测量含有水凝胶的滑液的粘度，以及在下沉溶解试验中使用可水解酯键测量与阳离子取代的葡聚糖缀合的模型活性物质的释放速率。使用荧光分子断层扫描在体内测量关节内注射到大鼠膝关节后的纳米颗粒保留。从离子交联的水凝胶在滑液中形成的阳离子纳米粒子的扩散和对流运输在体外比不带电的纳米粒子慢。纳米颗粒与滑液混合后形成的水凝胶在体外没有明显改变滑液的粘度。缀合肽从阳离子纳米颗粒的体外释放约为每周20%。在大鼠膝关节内注射后，70%的纳米颗粒保留在关节中1周。该研究证明了使用阳离子聚合物纳米颗粒增加治疗剂在关节中的保留以用于诸如骨关节炎的适应症的可行性。
To evaluate using cationic polymeric nanoparticles that interact with hyaluronate to form ionically cross-linked hydrogels to increase the intra-articular retention time of osteoarthritis drugs in the synovial cavity. In vitro tests included nanoparticle release from cross-linked hydrogels using syringe and membrane dissolution tests, viscosity measurement of synovial fluid containing hydrogels, and release-rate measurement for a model active conjugated to a cationically substituted dextran using a hydrolyzable ester linkage in a sink dissolution test. Nanoparticle retention after intra-articular injection into rat knees was measured in vivo using fluorescence molecular tomography. Diffusional and convective transport of cationic nanoparticles from ionically cross-linked hydrogels formed in synovial fluid was slower in vitro than for uncharged nanoparticles. Hydrogels formed after the nanoparticles were mixed with synovial fluid did not appreciably alter the viscosity of the synovial fluid in vitro. In vitro release of a conjugated peptide from the cationic nanoparticles was approximately 20% per week. After intra-articular injection in rat knees, 70% of the nanoparticles were retained in the joint for 1 week. This study demonstrates the feasibility of using cationic polymeric nanoparticles to increase the retention of therapeutic agents in articular joints for indications such as osteoarthritis.
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影响因子：
4.4
作者：
Edwards, Scott H. R.;Cake, Martin A.;Read, Richard A.
通讯作者：
Read, Richard A.
影响因子：
16.1
作者：
LARSEN C
通讯作者：
LARSEN C
影响因子：
--
作者：
Hellstern, Daniel;Schulze, Katia;von Rechenberg, Brigitte
通讯作者：
von Rechenberg, Brigitte
影响因子：
4
作者：
BONANOMI, MH;VELVART, M;WEDER, HG
通讯作者：
WEDER, HG
影响因子：
3.3
作者：
RATCLIFFE, JH;HUNNEYBALL, IM;DAVIS, SS
通讯作者：
DAVIS, SS