The yeast Red1 protein localizes to the cores of meiotic chromosomes.
The yeast Red1 protein localizes to the cores of meiotic chromosomes.
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DOI：
10.1083/jcb.136.5.957
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发表时间：
1997-03-10
影响因子：
7.8
通讯作者：
Roeder, GS
中科院分区：
文献类型：
--
作者：
Smith, AV;Roeder, GS
减数分裂特异性RED 1基因的突变体。酿酒酵母不能制造任何联会复合体（SC）或任何明显的SC前体。使用特异性识别Red 1蛋白的抗体，Red 1已被定位于沿着减数分裂粗线期染色体。Red 1还定位于zip 1突变体中存在的非突触轴向元件，这表明Red 1是成熟SC侧向元件的组成部分。抗Red 1染色仅限于减数分裂染色体的核心，是不相关的染色质的环路，位于SC外的spo 11突变体的分析表明，Red 1本地化不依赖于减数分裂重组。Red 1的定位已经与染色体的其他两个减数分裂特异性组分Hop 1和Zip 1进行了比较; Zip 1作为突触染色体的标记。用抗Zip 1和抗Red 1抗体对野生型减数分裂染色体进行双标记，表明Red 1在粗线期之前和期间都定位于染色体。双标记与抗Hop 1和抗Red 1抗体显示，Hop 1蛋白定位仅在区域，也包含Red 1，和Hop 1定位在一个red 1无效突变体的研究表明，Hop 1定位依赖于Red 1功能。这些观察结果与先前的遗传研究一致，表明Red 1和Hop 1直接相互作用。在粗线期染色体或突触染色体区域几乎没有Hop 1蛋白。
Mutants in the meiosis-specific RED1 gene of S. cerevisiae fail to make any synaptonemal complex (SC) or any obvious precursors to the SC. Using antibodies that specifically recognize the Red1 protein, Red1 has been localized along meiotic pachytene chromosomes. Red1 also localizes to the unsynapsed axial elements present in a zip1 mutant, suggesting that Red1 is a component of the lateral elements of mature SCs. Anti-Red1 staining is confined to the cores of meiotic chromosomes and is not associated with the loops of chromatin that lie outside the SC. Analysis of the spo11 mutant demonstrates that Red1 localization does not depend upon meiotic recombination. The localization of Red1 has been compared with two other meiosisspecific components of chromosomes, Hop1 and Zip1; Zip1 serves as a marker for synapsed chromosomes. Double labeling of wild-type meiotic chromosomes with anti-Zip1 and anti-Red1 antibodies demonstrates that Red1 localizes to chromosomes both before and during pachytene. Double labeling with anti-Hop1and anti-Red1 antibodies reveals that Hop1 protein localizes only in areas that also contain Red1, and studies of Hop1 localization in a red1 null mutant demonstrate that Hop1 localization depends on Red1 function. These observations are consistent with previous genetic studies suggesting that Red1 and Hop1 directly interact. There is little or no Hop1 protein on pachytene chromosomes or in synapsed chromosomal regions.
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Rap1和拓扑异构酶II在酵母的核和减数分裂染色体中的定位。
影响因子：
7.8
作者：
Klein, F;Laroche, T;Cardenas, M E;Hofmann, J F;Schweizer, D;Gasser, S M
通讯作者：
Gasser, S M
影响因子：
2.9
作者：
GUAN, KL;DIXON, JE
通讯作者：
DIXON, JE
DOI：
10.1073/pnas.74.11.5091
发表时间：
1977-01-01
影响因子：
11.1
作者：
PETES, TD;BOTSTEIN, D
通讯作者：
BOTSTEIN, D
影响因子：
10.5
作者：
ROCKMILL, B;SYM, M;ROEDER, GS
通讯作者：
ROEDER, GS
影响因子：
2.6
作者：
HILL, JE;MYERS, AM;TZAGOLOFF, A
通讯作者：
TZAGOLOFF, A