Host innate and adaptive immunity shapes the gut microbiota biogeography.
Host innate and adaptive immunity shapes the gut microbiota biogeography.
复制标题
宿主的先天免疫和获得性免疫塑造了肠道微生物区系的生物地理学。
DOI：
10.1111/1348-0421.12963
复制
发表时间：
2022-06
影响因子：
2.6
通讯作者：
Welsh, David A.
中科院分区：
文献类型：
--
作者：
Gu, Min;Samuelson, Derrick R.;de la Rua, Nicholas M.;Charles, Tysheena P.;Taylor, Christopher M.;Luo, Meng;Siggins, Robert W.;Shellito, Judd E.;Welsh, David A.
肠道微生物群在宿主免疫系统的发育和成熟中起着重要作用。先天性和适应性免疫细胞在微生物病原体遏制和清除方面都具有关键功能，但这些主要免疫细胞群体对胃肠道中肠道微生物组生态系统的调节尚未完全确定。我们通过免疫地理学研究了特异性先天性和适应性免疫细胞在肠道微生物群调节中的作用。树突状细胞，巨噬细胞，CD 4 + T细胞，CD 8 + T细胞，和B细胞使用单克隆抗体和氯膦酸盐脂质体耗尽，和微生物群落通过16 S rRNA基因测序确定。随着特异性免疫细胞耗竭，观察到明显的微生物群变化。一般来说，免疫细胞耗尽的小鼠在所有肠道解剖部位具有更高的微生物群丰富度和均匀度。在每个肠段，来自免疫细胞耗尽动物的样品远离同种型/脂质体对照小鼠聚集。这对于小肠微生物群尤其显著。具体而言，肠杆菌科、产酸拟杆菌和Schaedleri黏菌在免疫细胞缺陷动物的小肠粘膜和管腔中高度富集。此外，在所有肠段处，与管腔微生物群相比，粘膜微生物群具有更高的微生物群均匀度，并且B细胞耗尽和同种型对照小鼠之间的UniFrac距离在十二指肠中最大，其次是回肠和结肠。综上所述，我们的数据表明，先天性和适应性免疫细胞特别有助于调节肠道微生物群沿胃肠道沿着的地理分布，并且与其他解剖部位相比，十二指肠粘膜中的微生物群对宿主免疫变化的反应更强。
The gut microbiota has a fundamental role in the development and the maturation of the host immune system. Both innate and adaptive immune cells have critical functions in microbial pathogen containment and clearance, but the regulation of the commensal microbiome ecosystem in the gastrointestinal tract by these major immune cell populations is incompletely defined. We investigated the role of specific innate and adaptive immune cell in the regulation of the microbiota in the intestinal tract biogeographically. Dendritic cells, macrophages, CD4+ T-cells, CD8+ T-cells, and B-cells were depleted using monoclonal antibodies and clodronate liposomes, and the microbial communities was determined by 16S rRNA gene sequencing. With specific immune cell depletion, distinct microbiota changes were observed. In general, immune cell depleted mice had higher microbiota richness and evenness at all gut anatomical sites. At each gut segment, samples from immune cell-depleted animals clustered away from the Isotype/Liposome control mice. This was especially dramatic for small intestinal microbiota. Specifically, Enterobacteriaceae, Bacteroides acidifaciens and Mucispirillum schaedleri were highly enriched in the mucosa and lumen of the small intestine in immune cell-deficient animals. Further, the mucosal microbiota had higher microbiota evenness compared to luminal microbiota at all gut segments, and the UniFrac distance between B cell depleted and isotype control mice was the largest in duodenum followed by ileum and colon. Taken together, our data suggest that innate and adaptive immune cells specifically contribute to the regulation of the gut microbiota’s biogeographical distribution along the gastrointestinal tract, and microbiota in duodenum mucosa are more responsive to host immune changes compared to other anatomical sites.
登录
查看更多内容
影响因子：
16.6
作者：
Li H;Limenitakis JP;Fuhrer T;Geuking MB;Lawson MA;Wyss M;Brugiroux S;Keller I;Macpherson JA;Rupp S;Stolp B;Stein JV;Stecher B;Sauer U;McCoy KD;Macpherson AJ
通讯作者：
Macpherson AJ
DOI：
10.4049/jimmunol.1302884
发表时间：
2014-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Han S;Lin YC;Wu T;Salgado AD;Mexhitaj I;Wuest SC;Romm E;Ohayon J;Goldbach-Mansky R;Vanderver A;Marques A;Toro C;Williamson P;Cortese I;Bielekova B
通讯作者：
Bielekova B
影响因子：
--
作者：
Koscso, Balazs;Bogunovic, Milena
通讯作者：
Bogunovic, Milena
DOI：
10.1152/ajpgi.00029.2015
发表时间：
2015-05-15
影响因子：
4.5
作者：
Hamilton, M. Kristina;Boudry, Galle;Raybould, Helen E.
通讯作者：
Raybould, Helen E.
影响因子：
64.8
作者：
Bouskra, Djahida;Brezillon, Christophe;Eberl, Gerard
通讯作者：
Eberl, Gerard