Noninvasive, in vivo assessment of mouse retinal structure using optical coherence tomography.
Noninvasive, in vivo assessment of mouse retinal structure using optical coherence tomography.
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DOI：
10.1371/journal.pone.0007507
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发表时间：
2009-10-19
期刊：
影响因子：
3.7
通讯作者：
Seeliger MW
中科院分区：
文献类型：
--
作者：
Fischer MD;Huber G;Beck SC;Tanimoto N;Muehlfriedel R;Fahl E;Grimm C;Wenzel A;Remé CE;van de Pavert SA;Wijnholds J;Pacal M;Bremner R;Seeliger MW
光学相干层析成像（OCT）是一种新型的视网膜活体成像方法。在这项研究中，我们评估了OCT在视网膜变性小鼠模型中产生组织学模拟切片的潜力。我们实现了适应商业第三代OCT系统，以获得和量化迄今为止需要体外组织学的小鼠视网膜的高分辨率形态切片。在发育缺陷、光损伤和遗传性视网膜变性模型中比较OCT和组织学。在视网膜成视网膜细胞瘤蛋白Rb缺陷的条件性基因敲除小鼠中，Cre表达从中心到周边的梯度导致视网膜厚度逐渐减少，清晰可见，并且可以很好地进行地形定量。在Nrl敲除小鼠中，参与玫瑰花结样结构形成的层与组织学中类似地清楚。通过自体荧光模式很好地划分的焦点光损伤的OCT检查显示光感受器几乎完全丧失，保留了视网膜内层，但也有更细微的变化，如水肿形成。在Crb1基因敲除小鼠（Leber先天性黑蒙模型）中，可以很好地识别由于感光细胞内节的亚顶端区域缺乏粘附而导致的视网膜血管穿过外核层滑向视网膜色素上皮（RPE）。我们发现，使用OCT，我们能够检测和分析广泛的小鼠视网膜病变，并且结果与组织切片进行了很好的比较。此外，该技术允许随时间推移跟踪单个动物，从而减少所需的研究动物数量，并评估动态过程，如水肿形成。结果清楚地表明，OCT有可能彻底改变各自的短期和长期研究的未来设计，以及治疗策略的临床前评估。
Optical coherence tomography (OCT) is a novel method of retinal in vivo imaging. In this study, we assessed the potential of OCT to yield histology-analogue sections in mouse models of retinal degeneration. We achieved to adapt a commercial 3rd generation OCT system to obtain and quantify high-resolution morphological sections of the mouse retina which so far required in vitro histology. OCT and histology were compared in models with developmental defects, light damage, and inherited retinal degenerations. In conditional knockout mice deficient in retinal retinoblastoma protein Rb, the gradient of Cre expression from center to periphery, leading to a gradual reduction of retinal thickness, was clearly visible and well topographically quantifiable. In Nrl knockout mice, the layer involvement in the formation of rosette-like structures was similarly clear as in histology. OCT examination of focal light damage, well demarcated by the autofluorescence pattern, revealed a practically complete loss of photoreceptors with preservation of inner retinal layers, but also more subtle changes like edema formation. In Crb1 knockout mice (a model for Leber's congenital amaurosis), retinal vessels slipping through the outer nuclear layer towards the retinal pigment epithelium (RPE) due to the lack of adhesion in the subapical region of the photoreceptor inner segments could be well identified. We found that with the OCT we were able to detect and analyze a wide range of mouse retinal pathology, and the results compared well to histological sections. In addition, the technique allows to follow individual animals over time, thereby reducing the numbers of study animals needed, and to assess dynamic processes like edema formation. The results clearly indicate that OCT has the potential to revolutionize the future design of respective short- and long-term studies, as well as the preclinical assessment of therapeutic strategies.
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影响因子：
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作者：
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