Quantifying 3D Strain in Scaffold Implants for Regenerative Medicine.
Quantifying 3D Strain in Scaffold Implants for Regenerative Medicine.
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DOI：
10.3390/ma13173890
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发表时间：
2020-09-03
期刊：
影响因子：
--
通讯作者：
Jeffers JRT
中科院分区：
文献类型：
--
作者：
Clark JN;Tavana S;Heyraud A;Tallia F;Jones JR;Hansen U;Jeffers JRT
再生医学解决方案需要深思熟虑的设计，以引起预期的生物反应。这包括生物力学刺激，以在支架和周围组织中产生适当的应变，从而将细胞谱系驱动到所需的组织。为了在局部水平上提供适当的应变，新一代支架通常涉及各向异性的空间分级机械性能，这些性能无法用传统的材料测试设备表征。通过三维（3D）成像、原位加载和数字容积相关（DVC）进行容积检查是可能的。在这项研究中，Micro-CT和DVC被用于两种尺寸的3D打印无机/有机混合支架（n = 2和n = 4），其具有用于软骨再生的重复均匀结构。变形的空间分辨率低于200 µm，同时保持位移随机误差为0.97 µm，应变系统误差为0.17%，应变随机误差为0.031%。数字图像相关（DIC）提供了外表面的分析，而DVC使局部应变集中在整个3D体积进行检查。使用DVC得出的应变值与手动计算的地面实况测量值相关性良好（R2 = 0.98，n = 8）。该技术确保细胞所经历的完整3D微机械环境得到密切考虑，使未来的研究能够进一步研究再生医学的支架设计。
Regenerative medicine solutions require thoughtful design to elicit the intended biological response. This includes the biomechanical stimulus to generate an appropriate strain in the scaffold and surrounding tissue to drive cell lineage to the desired tissue. To provide appropriate strain on a local level, new generations of scaffolds often involve anisotropic spatially graded mechanical properties that cannot be characterised with traditional materials testing equipment. Volumetric examination is possible with three-dimensional (3D) imaging, in situ loading and digital volume correlation (DVC). Micro-CT and DVC were utilised in this study on two sizes of 3D-printed inorganic/organic hybrid scaffolds (n = 2 and n = 4) with a repeating homogenous structure intended for cartilage regeneration. Deformation was observed with a spatial resolution of under 200 µm whilst maintaining displacement random errors of 0.97 µm, strain systematic errors of 0.17% and strain random errors of 0.031%. Digital image correlation (DIC) provided an analysis of the external surfaces whilst DVC enabled localised strain concentrations to be examined throughout the full 3D volume. Strain values derived using DVC correlated well against manually calculated ground-truth measurements (R2 = 0.98, n = 8). The technique ensures the full 3D micro-mechanical environment experienced by cells is intimately considered, enabling future studies to further examine scaffold designs for regenerative medicine.
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DOI：
10.1016/j.jmbbm.2018.08.012
发表时间：
2018-12-01
影响因子：
3.9
作者：
Fernandez, Marta Peiia;Cipiccia, Silvia;Tozzi, Gianluca
通讯作者：
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影响因子：
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2.2
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影响因子：
1.9
作者：
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影响因子：
2
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