Evidence of non-Plasmodium falciparum malaria infection in Kédougou, Sénégal.
Evidence of non-Plasmodium falciparum malaria infection in Kédougou, Sénégal.
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DOI：
10.1186/s12936-016-1661-3
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发表时间：
2017-01-03
期刊：
影响因子：
3
通讯作者：
Ndiaye D
中科院分区：
文献类型：
--
作者：
Daniels RF;Deme AB;Gomis JF;Dieye B;Durfee K;Thwing JI;Fall FB;Ba M;Ndiop M;Badiane AS;Ndiaye YD;Wirth DF;Volkman SK;Ndiaye D
在塞内加尔扩大疟疾防治工作后，更多地使用快速诊断测试来确定主要致病疟原虫种类-恶性疟原虫。然而，在塞内加尔使用的RDT类型不能检测其他引起疟疾的物种，如卵形疟原虫，三日疟原虫或间日疟原虫。因此，缺乏关于塞内加尔疟疾感染频率和类型的信息。这项研究旨在更好地确定在塞内加尔Kédougou评估可能的疟疾感染的患者中是否存在恶性疟原虫以外的物种。间日疟原虫、卵形疟原虫属种的实时聚合酶链反应形态分析，和三日疟原虫通过测序进行了开发和验证，并对2013年至2014年期间从塞内加尔东南部Kédougou的两个卫生诊所的症状患者的设施样本中收集的475个基于恶性疟原虫特异性HRP 2的RDT中提取的DNA进行了分析。 在RDT结果阳性的患者（n = 187）中，观察到三日疟原虫（n = 3）和卵形疟原虫（n = 2）与恶性疟原虫合并感染，其中1例患者对所有三种疟原虫均呈阳性。在288份阴性RDT样本中，鉴定出恶性疟原虫（n = 24）、卵形疟原虫（n = 3）、卵形疟原虫（n = 1）和三日疟原虫（n = 3）阳性样本，对应于2.5%的非恶性疟原虫阳性率。这些发现强调了RDT用于疟疾诊断的局限性，并表明塞内加尔发生了非恶性疟原虫疟疾感染。目前用于常规临床诊断的快速诊断试验不一定能准确反映塞内加尔凯杜古的疟疾传播情况，诊断和病人护理以及监测和消灭活动需要更敏感和更具体的方法。这些发现对其他疟疾流行环境具有影响，在这些环境中，除恶性疟原虫外的其他物种可能被传播，并被控制或消灭活动所忽视。本文的在线版本（doi：10.1186/s12936-016-1661-3）包含补充材料，可供授权用户使用。
Expanded malaria control efforts in Sénégal have resulted in increased use of rapid diagnostic tests (RDT) to identify the primary disease-causing Plasmodium species, Plasmodium falciparum. However, the type of RDT utilized in Sénégal does not detect other malaria-causing species such as Plasmodium ovale spp., Plasmodium malariae, or Plasmodium vivax. Consequently, there is a lack of information about the frequency and types of malaria infections occurring in Sénégal. This study set out to better determine whether species other than P. falciparum were evident among patients evaluated for possible malaria infection in Kédougou, Sénégal. Real-time polymerase chain reaction speciation assays for P. vivax, P. ovale spp., and P. malariae were developed and validated by sequencing and DNA extracted from 475 Plasmodium falciparum-specific HRP2-based RDT collected between 2013 and 2014 from a facility-based sample of symptomatic patients from two health clinics in Kédougou, a hyper-endemic region in southeastern Sénégal, were analysed. Plasmodium malariae (n = 3) and P. ovale wallikeri (n = 2) were observed as co-infections with P. falciparum among patients with positive RDT results (n = 187), including one patient positive for all three species. Among 288 negative RDT samples, samples positive for P. falciparum (n = 24), P. ovale curtisi (n = 3), P. ovale wallikeri (n = 1), and P. malariae (n = 3) were identified, corresponding to a non-falciparum positivity rate of 2.5%. These findings emphasize the limitations of the RDT used for malaria diagnosis and demonstrate that non-P. falciparum malaria infections occur in Sénégal. Current RDT used for routine clinical diagnosis do not necessarily provide an accurate reflection of malaria transmission in Kédougou, Sénégal, and more sensitive and specific methods are required for diagnosis and patient care, as well as surveillance and elimination activities. These findings have implications for other malaria endemic settings where species besides P. falciparum may be transmitted and overlooked by control or elimination activities. The online version of this article (doi:10.1186/s12936-016-1661-3) contains supplementary material, which is available to authorized users.
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影响因子：
3.8
作者：
Miller RH;Obuya CO;Wanja EW;Ogutu B;Waitumbi J;Luckhart S;Stewart VA
通讯作者：
Stewart VA
影响因子：
3
作者：
Alemu, Abebe;Fuehrer, Hans-Peter;Noedl, Harald
通讯作者：
Noedl, Harald
DOI：
10.1073/pnas.1505691112
发表时间：
2015-06-02
影响因子：
11.1
作者：
Daniels, Rachel F.;Schaffner, Stephen F.;Wirth, Dyann F.
通讯作者：
Wirth, Dyann F.
影响因子：
3
作者：
Daniels, Rachel;Volkman, Sarah K.;Wiegand, Roger C.
通讯作者：
Wiegand, Roger C.
影响因子：
4
作者：
Oguike MC;Betson M;Burke M;Nolder D;Stothard JR;Kleinschmidt I;Proietti C;Bousema T;Ndounga M;Tanabe K;Ntege E;Culleton R;Sutherland CJ
通讯作者：
Sutherland CJ