Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production.
Comparing the fermentation performance of Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST) and Zymomonas mobilis AX101 for cellulosic ethanol production.
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DOI：
10.1186/1754-6834-3-11
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发表时间：
2010-05-27
影响因子：
6.3
通讯作者：
Dale BE
中科院分区：
文献类型：
--
作者：
Lau MW;Gunawan C;Balan V;Dale BE
在玉米浸泡液(CSL)和氨纤维膨化(AFEX)处理的玉米秸秆水提物和酶解物上，比较了大肠杆菌KO11、酿酒酵母424A(LNH-ST)和运动发酵单胞菌AX101的发酵效果。这三种乙醇源在添加CSL的联合发酵中能够以大于0.42g/g消耗糖、40g/L和0.7g/L/h(0~48h)的代谢产率、最终浓度和速率产生乙醇。在CSL发酵中，所测试的产乙醇菌的纯木糖发酵速度是424A(LNH-ST)的5-8倍。所有受试菌株都在15%w/v固体负荷下生长和共同发酵糖，固体负荷相当于氨纤维爆炸(AFEX)预处理的玉米秸秆水提取物。然而，KO11和424A(LNH-ST)都表现出比AX101更强的生长健壮性。在装载量为18%w/w的木质纤维素水解液中，葡萄糖的完全发酵速率大于0.77g/L/h。与CSL发酵结果相比，酿酒酵母424A(LNH-ST)在水解液中消耗木糖的幅度和速率最大。我们的结果证实，被测试菌株之间的葡萄糖发酵即使在高固体负荷(18%重量)下也是有效的。然而，木质纤维水解液中木糖的消耗是影响木质纤维水解液总得率、效价或速率的主要瓶颈。相比之下，酿酒酵母424A(LNH-ST)是最适合工业化生产的菌株，因为它能够从AFEX处理的玉米秸秆的未解毒和未添加的水解液中高产地发酵葡萄糖和木糖。
Fermentations using Escherichia coli KO11, Saccharomyces cerevisiae 424A(LNH-ST), and Zymomonas mobilis AX101 are compared side-by-side on corn steep liquor (CSL) media and the water extract and enzymatic hydrolysate from ammonia fiber expansion (AFEX)-pretreated corn stover. The three ethanologens are able produce ethanol from a CSL-supplemented co-fermentation at a metabolic yield, final concentration and rate greater than 0.42 g/g consumed sugars, 40 g/L and 0.7 g/L/h (0-48 h), respectively. Xylose-only fermentation of the tested ethanologenic bacteria are five to eight times faster than 424A(LNH-ST) in the CSL fermentation. All tested strains grow and co-ferment sugars at 15% w/v solids loading equivalent of ammonia fiber explosion (AFEX)-pretreated corn stover water extract. However, both KO11 and 424A(LNH-ST) exhibit higher growth robustness than AX101. In 18% w/w solids loading lignocellulosic hydrolysate from AFEX pretreatment, complete glucose fermentations can be achieved at a rate greater than 0.77 g/L/h. In contrast to results from fermentation in CSL, S. cerevisiae 424A(LNH-ST) consumed xylose at the greatest extent and rate in the hydrolysate compared to the bacteria tested. Our results confirm that glucose fermentations among the tested strains are effective even at high solids loading (18% by weight). However, xylose consumption in the lignocellulosic hydrolysate is the major bottleneck affecting overall yield, titer or rate of the process. In comparison, Saccharomyces cerevisiae 424A(LNH-ST) is the most relevant strains for industrial production for its ability to ferment both glucose and xylose from undetoxified and unsupplemented hydrolysate from AFEX-pretreated corn stover at high yield.
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影响因子：
4.4
作者：
OHTA, K;BEALL, DS;INGRAM, LO
通讯作者：
INGRAM, LO
影响因子：
3.8
作者：
Lau, Ming W.;Dale, Bruce E.;Balan, Venkatesh
通讯作者：
Balan, Venkatesh
影响因子：
3
作者：
Teymouri, F;Laureano-Pérez, L;Dale, BE
通讯作者：
Dale, BE
影响因子：
3
作者：
Sedlak, M;Ho, NWY
通讯作者：
Ho, NWY
影响因子：
2.7
作者：
Moniruzzaman, M;Ingram, LO
通讯作者：
Ingram, LO