Molecular analysis of the short arm of chromosome 3 in small-cell and non-small-cell carcinoma of the lung.-猫眼课题宝

小细胞和非小细胞肺癌中 3 号染色体短臂的分子分析。

DOI：

10.1056/nejm198710293171803

发表时间：

1987

期刊：

The New England journal of medicine

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

H. Brauch;Bruce E. Johnson;Janet Hovis;Takahiko Yano;A. Gazdar;Olive S. Pettengill;Stephen L. Graziano;George D. Sorenson;Bernard J. Poiesz;J. Minna;Marston Linehan;Berton Zbar

以往的研究表明，3号染色体短臂(3p)上的DNA序列缺失与小细胞肺癌有关。因此，我们在42例小细胞或非小细胞肺癌患者的肿瘤组织中寻找3p等位基因的缺失。13例小细胞肺癌患者在正常肺组织中存在一个或多个3p等位基因杂合子时，肿瘤组织中至少有一个共显性等位基因丢失。来自另外8个患者的小细胞肺癌细胞系的3p等位基因纯合；这一结果与预测的纯合频率显著不同。研究的肿瘤组织包括从活检标本中获得的小细胞肺癌细胞系，尸检样本，以及含有肿瘤细胞的切除的淋巴。在化疗前后的肿瘤标本中观察到3p等位基因丢失。在15例可评估的非小细胞肺癌患者中，有4例存在3p等位基因缺失。我们得出结论，3p等位基因缺失是一种在小细胞肺癌中持续存在的变化，偶尔在非小细胞肺癌中也存在。

Previous studies have suggested that the loss of DNA sequences on the short arm of chromosome 3 (3p) is associated with small-cell lung carcinoma. We therefore looked for loss of 3p alleles in tumor tissue from 42 patients with either small-cell or non-small-cell lung carcinoma. All 13 patients with small-cell lung carcinoma who were heterozygous for one or more alleles at 3p in normal tissue had the loss of at least one codominant allele in the tumor tissue. Cell lines of small-cell lung carcinoma from an additional eight patients were homozygous for 3p alleles; this result was significantly different from the predicted frequency of homozygosity. The tumor tissue studied included cell lines of small-cell lung carcinoma obtained from biopsy specimens, an autopsy sample, and an excised lymph node containing tumor cells. Loss of alleles at 3p was observed in tumor samples obtained before and after chemotherapy. Four of 15 evaluable patients with non-small-cell carcinoma of the lung had loss of 3p alleles. We conclude that loss of alleles at 3p is a change found consistently in small-cell lung carcinoma and occasionally in non-small-cell lung carcinoma.

Hemizygous interstitial deletion of chromosome 15 (band D) in three translocation-negative murine plasmacytomas.