A bacterial extracellular DNA inhibits settling of motile progeny cells within a biofilm.
A bacterial extracellular DNA inhibits settling of motile progeny cells within a biofilm.
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DOI：
10.1111/j.1365-2958.2010.07267.x
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发表时间：
2010-08
影响因子：
3.6
通讯作者：
Brun YV
中科院分区：
文献类型：
--
作者：
Berne C;Kysela DT;Brun YV
在自然系统中，细菌形成复杂的表面附着群落，称为生物膜。与独立或浮游生物相比，这种生活方式具有许多优点，例如交换营养物质，免受环境压力的影响，以及对杀菌剂的耐受性增强。尽管有这些好处，但在逃离恶化的环境和成功殖民有利的、无人居住的栖息地斑块方面，扩散也起着重要作用。α-弯茎杆菌在每次细胞分裂时产生一个可运动的簇生细胞和一个无根的柄状细胞。我们在这里表明，月牙菇细胞外DNA （eDNA）抑制其运动细胞类型在生物膜中定居的能力。eDNA与极性固定物结合，这是一种永久表面附着和生物膜形成所需的粘附结构，从而抑制细胞附着。由于跟踪细胞通过其固结物与生物膜紧密结合，我们假设这种新机制作用于在生物膜中出生的群细胞，在那里eDNA可以积累到足够的浓度来抑制它们的沉降能力。通过靶向生物膜中的特定细胞类型，该机制调节生物膜的发育并促进扩散，而不会导致现有生物膜的潜在不良溶解。
In natural systems, bacteria form complex, surface-attached communities known as biofilms. This lifestyle presents numerous advantages compared to unattached or planktonic life, such as exchange of nutrients, protection from environmental stresses and increased tolerance to biocides. Despite such benefits, dispersal also plays an important role in escaping deteriorating environments and in successfully colonizing favorable, unoccupied habitat patches. The α-proteobacterium Caulobacter crescentus produces a motile swarmer cell and a sessile stalked cell at each cell division. We show here that C. crescentus extracellular DNA (eDNA) inhibits the ability of its motile cell type to settle in a biofilm. eDNA binds to the polar holdfast, an adhesive structure required for permanent surface attachment and biofilm formation, thereby inhibiting cell attachment. Since stalked cells associate tightly with the biofilm through their holdfast, we hypothesize that this novel mechanism acts on swarmer cells born in a biofilm, where eDNA can accumulate to a sufficient concentration to inhibit their ability to settle. By targeting a specific cell type in a biofilm, this mechanism modulates biofilm development and promotes dispersal without causing a potentially undesirable dissolution of the existing biofilm.
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影响因子：
3.2
作者：
Bodenmiller, D;Toh, E;Brun, YV
通讯作者：
Brun, YV
影响因子：
3.2
作者：
Jurcisek, Joseph A.;Bakaletz, Lauren O.
通讯作者：
Bakaletz, Lauren O.
影响因子：
3.6
作者：
Allesen-Holm, M;Barken, KB;Tolker-Nielsen, T
通讯作者：
Tolker-Nielsen, T
影响因子：
5.6
作者：
DEGNEN, ST;NEWTON, A
通讯作者：
NEWTON, A
影响因子：
3.2
作者：
Ackerley, DF;Barak, Y;Matin, A
通讯作者：
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