The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism.
The EAL-domain protein FcsR regulates flagella, chemotaxis and type III secretion system in Pseudomonas aeruginosa by a phosphodiesterase independent mechanism.
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DOI：
10.1038/s41598-017-09926-3
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发表时间：
2017-08-31
影响因子：
4.6
通讯作者：
Durán R
中科院分区：
文献类型：
--
作者：
Rossello J;Lima A;Gil M;Rodríguez Duarte J;Correa A;Carvalho PC;Kierbel A;Durán R
第二信使c-di-GMP调节能动和固着细菌生活方式之间的转换。c-di-GMP代谢的一般特征是存在令人惊讶的大量编码二鸟苷酸环化酶和磷酸二酯酶（分别负责其合成和降解的酶）的基因。然而，这种明显的冗余的生理相关性尚不清楚，强调需要调查这些酶的功能。本文主要研究铜绿假单胞菌的磷酸二酯酶PA 2133。我们对铜绿假单胞菌菌株K过表达PA 2133或其失活突变体进行了表型表征。我们发现，生物膜的形成和运动性严重受损的过度表达的PA 2133。我们的定量蛋白质组学方法应用于膜和外蛋白组分显示，参与三个过程的蛋白质主要受到影响：鞭毛运动，III型分泌系统和趋化性。虽然生物膜形成的抑制可归因于PA 2133的磷酸二酯酶活性，但鞭毛、趋化性和III型分泌系统蛋白的下调与该酶活性无关。基于PA 2133的这些意想不到的作用，我们建议将该基因产物重命名为FcsR，即鞭毛、趋化性和III型分泌系统调节剂。
The second messenger c-di-GMP regulates the switch between motile and sessile bacterial lifestyles. A general feature of c-di-GMP metabolism is the presence of a surprisingly large number of genes coding for diguanylate cyclases and phosphodiesterases, the enzymes responsible for its synthesis and degradation respectively. However, the physiological relevance of this apparent redundancy is not clear, emphasizing the need for investigating the functions of each of these enzymes. Here we focused on the phosphodiesterase PA2133 from Pseudomonas aeruginosa, an important opportunistic pathogen. We phenotypically characterized P. aeruginosa strain K overexpressing PA2133 or its inactive mutant. We showed that biofilm formation and motility are severely impaired by overexpression of PA2133. Our quantitative proteomic approach applied to the membrane and exoprotein fractions revealed that proteins involved in three processes were mostly affected: flagellar motility, type III secretion system and chemotaxis. While inhibition of biofilm formation can be ascribed to the phosphodiesterase activity of PA2133, down-regulation of flagellar, chemotaxis, and type III secretion system proteins is independent of this enzymatic activity. Based on these unexpected effects of PA2133, we propose to rename this gene product FcsR, for Flagellar, chemotaxis and type III secretion system Regulator.
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影响因子：
4.2
作者：
Galperin MY
通讯作者：
Galperin MY
影响因子：
2.8
作者：
Davinic, Marko;Carty, Nancy L.;Hamood, Abdul N.
通讯作者：
Hamood, Abdul N.
DOI：
10.1038/nrmicro2199
发表时间：
2009-09
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.2
作者：
Barraud, Nicolas;Schleheck, David;Kjelleberg, Staffan
通讯作者：
Kjelleberg, Staffan
影响因子：
3.6
作者：
Borlee BR;Goldman AD;Murakami K;Samudrala R;Wozniak DJ;Parsek MR
通讯作者：
Parsek MR