A device for performing lateral conductance measurements on individual double-stranded DNA molecules.
A device for performing lateral conductance measurements on individual double-stranded DNA molecules.
复制标题
DOI：
10.1021/nn303322r
复制
发表时间：
2012-10-23
期刊：
影响因子：
17.1
通讯作者：
Ramsey, J. Michael
中科院分区：
文献类型：
--
作者：
Menard, Laurent D.;Mair, Chad E.;Woodson, Michael E.;Alarie, Jean Pierre;Ramsey, J. Michael
描述了一种能够电监测DNA分子通过纳米通道的驱动移位的纳米流体装置。这是通过将长的传输通道与较短的正交纳米通道相交来实现的。该横向纳米通道的离子电导进行监测，而DNA是电动驱动通过运输通道。当DNA通过交叉点时，横向电导发生改变，导致瞬态电流响应。在1 M KCl溶液中，发现相对于基线横向离子电流，这是5- 25%的电流增强。研究了两种不同的器械几何形状。在一个装置中，DNA在完全插入并通过运输纳米通道易位后被检测到。在另一个设备中，DNA在进入纳米通道的过程中被检测到。结果发现，这两种条件的特点是不同的传输动力学。同时光学和电学监测的DNA易位证实，瞬态事件起源于DNA运输通过纳米通道交叉。
A nanofluidic device is described that is capable of electrically monitoring the driven translocation of DNA molecules through a nanochannel. This is achieved by intersecting a long transport channel with a shorter orthogonal nanochannel. The ionic conductance of this transverse nanochannel is monitored while DNA is electrokinetically driven through the transport channel. When DNA passes the intersection, the transverse conductance is altered, resulting in a transient current response. In 1 M KCl solutions, this was found to be a current enhancement of 5–25%, relative to the baseline transverse ionic current. Two different device geometries were investigated. In one device, the DNA was detected after it was fully inserted into and translocating through the transport nanochannel. In the other device, the DNA was detected while it was in the process of entering the nanochannel. It was found that these two conditions are characterized by different transport dynamics. Simultaneous optical and electrical monitoring of DNA translocation confirmed that the transient events originated from DNA transport through the nanochannel intersection.
登录
查看更多内容
影响因子：
14.9
作者：
Günther K;Mertig M;Seidel R
通讯作者：
Seidel R
影响因子：
5.5
作者：
Balducci, Anthony;Mao, Pan;Doyle, Patrick S.
通讯作者：
Doyle, Patrick S.
影响因子：
3.4
作者：
Mannion, J. T.;Reccius, C. H.;Craighead, H. G.
通讯作者：
Craighead, H. G.
影响因子：
3.4
作者：
Bates, M;Burns, M;Meller, A
通讯作者：
Meller, A
影响因子：
10.8
作者：
Fischbein, Michael D.;Drndic, Marija
通讯作者：
Drndic, Marija