Nerve growth factor signaling following unilateral pelvic ganglionectomy in the rat ventral prostate is age dependent.
Nerve growth factor signaling following unilateral pelvic ganglionectomy in the rat ventral prostate is age dependent.
复制标题
大鼠腹侧前列腺单侧盆腔神经节切除术后的神经生长因子信号传导具有年龄依赖性。
DOI：
10.1038/aja.2013.59
复制
发表时间：
2013
影响因子：
2.9
通讯作者：
McVary,KevinT
中科院分区：
文献类型：
--
作者：
Podlasek,CarolA;Ghosh,Rudrani;OnurCakir,Omer;Bond,Christopher;McKenna,KevinE;McVary,KevinT
良性前列腺增生（BPH）是一个严重的健康问题，是许多男性下尿路症状（LUTS）的根本原因。在受影响的男性中，LUTS/BPH被认为是由前列腺的良性增殖导致膀胱出口梗阻引起的。出生后前列腺的生长是通过生长因子和内分泌机制控制的。然而，很少有人注意到自主神经系统在前列腺生长和分化中的作用。神经生长因子（NGF）是一种前列腺有丝分裂原，在自主感觉终末器官相互作用中具有营养作用。在这项研究中，我们研究了自主神经系统如何影响前列腺的生长作为年龄的函数，定量神经生长因子在大鼠腹侧前列腺（VP）后盆腔神经节切除术。出生后第30天（P30）、60天和120天的Sprague-Dawley大鼠与假手术对照组（n=39）进行单侧盆腔神经节切除术。术后7天，对失神经支配、完整（对侧）和假手术对照VP进行半定量RT-PCR、Western blotting和免疫组织化学分析。Ngf RNA表达在失神经支配和完整的增生VP中显著增加。免疫印迹显示，在对侧完整VP中，P60时NGF蛋白呈年龄依赖性增加。NGF定位于前列腺导管的神经、基底细胞和柱状上皮。去神经支配导致VP中NGF的年龄依赖性增加，这是自主神经系统可能调节前列腺生长并导致BPH/LUTS的潜在机制。
Benign prostatic hyperplasia (BPH) is a serious health concern and is an underlying cause of lower urinary tract symptoms (LUTS) in many men. In affected men, LUTS/BPH is believed to result from benign proliferation of the prostate resulting in bladder outlet obstruction. Postnatal growth of the prostate is controlled via growth factor and endocrine mechanisms. However, little attention had been given to the function of the autonomic nervous system in prostate growth and differentiation. Nerve growth factor (NGF) is a prostatic mitogen that has a trophic role in autonomic sensory end organ interaction. In this study, we examine how the autonomic nervous system influences prostate growth as a function of age by quantifying NGF in the rat ventral prostate (VP) after pelvic ganglionectomy. Unilateral pelvic ganglionectomy was performed on postnatal days 30 (P30), 60 and 120 Sprague–Dawley rats in comparison to sham controls (n=39). Semiquantitative RT-PCR, Western blotting and immunohistochemical analysis for NGF were performed on denervated, intact (contralateral side) and sham control VP 7 days after surgery. Ngf RNA expression was significantly increased in the denervated and intact hyperplastic VP. Western blotting showed age-dependent increases in NGF protein at P60 in the contralateral intact VP. NGF was localized in the nerves, basal cells and columnar epithelium of the prostatic ducts. Denervation causes age-dependent increases in NGF in the VP, which is a potential mechanism by which the autonomic nervous system may regulate prostate growth and lead to BPH/LUTS.
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神经营养蛋白分泌途径和突触可塑性
影响因子：
4.2
作者：
K. Lim;Seung T. Lim;H. Federoff
通讯作者：
H. Federoff
心脏神经生长因子表达的年龄相关区域差异
影响因子：
--
作者：
E. Saygili;R. Kluttig;O. Rana;E. Saygılı;C. Gemein;M. Zink;G. Rackauskas;J. Weis;R. Schwinger;N. Marx;P. Schauerte
通讯作者：
P. Schauerte
影响因子：
2.1
作者：
Girman, CJ;Jacobsen, SJ;Lieber, MM
通讯作者：
Lieber, MM
发育过程中接触雌激素会改变成年大鼠前列腺的上皮细胞粘附和间隙连接蛋白。
DOI：
10.1210/endo.142.1.7893
发表时间：
2001
期刊：
Endocrinology.
影响因子：
--
作者：
Habermann,H;Chang,WY;Birch,L;Mehta,P;Prins,GS
通讯作者：
Prins,GS
影响因子：
2.7
作者：
Risbridger, G;Wang, H;Cunha, G
通讯作者：
Cunha, G