No difference in keratin thickness between inner and outer foreskins from elective male circumcisions in Rakai, Uganda.
No difference in keratin thickness between inner and outer foreskins from elective male circumcisions in Rakai, Uganda.
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DOI：
10.1371/journal.pone.0041271
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hope TJ
中科院分区：
文献类型：
--
作者：
Dinh MH;Hirbod T;Kigozi G;Okocha EA;Cianci GC;Kong X;Prodger JL;Broliden K;Kaul R;Serwadda D;Wawer MJ;Gray RH;Hope TJ
据推测，未行包皮环切术的男性感染艾滋病毒的几率增加可能与内包皮角质化较薄有关。然而，已发表的数据相互矛盾，并可能受到包皮环切术医学适应症的混淆。我们测试的假设，内包皮角质化较薄，比外包皮组织从19个健康的，艾滋病毒未感染的男性进行常规预防性包皮环切术在Rakai，乌干达。将来自3个包皮解剖部位（内、外和系带）的切片分别快速冷冻。两个独立的实验室各自以盲法分别染色、成像和测量角蛋白厚度。内包皮（平均值= 14.67±7.48 µm）和外包皮（平均值= 13.30±8.49 µm）之间或内包皮和系带（平均值= 16.91±12.42 µm）之间的角蛋白厚度无显著差异。 虽然系带的角蛋白厚度表现出最大的个体内异质性，但在所有区域都存在大量的个体间差异。两个实验室的测量结果显示高度相关（r = 0.741，95%CI，0.533-0.864）。 我们的结论是，尽管个体间和个体内的差异，角蛋白厚度是相似的，在内部和外部包皮的健康乌干达男子，减少角蛋白厚度是不太可能使内部包皮更容易感染艾滋病毒的收购。
It has been hypothesized that increased HIV acquisition in uncircumcised men may relate to a more thinly keratinized inner foreskin. However, published data are contradictory and potentially confounded by medical indications for circumcision. We tested the hypothesis that the inner foreskin was more thinly keratinized than the outer foreskin using tissues from 19 healthy, HIV-uninfected men undergoing routine prophylactic circumcision in Rakai, Uganda. Sections from 3 foreskin anatomic sites (inner, outer, and frenar band) were snap-frozen separately. Two independent laboratories each separately stained, imaged, and measured keratin thicknesses in a blinded fashion. There was no significant difference in keratin thickness between the inner (mean = 14.67±7.48 µm) and outer (mean = 13.30±8.49 µm) foreskin, or between the inner foreskin and the frenar band (mean = 16.91±12.42 µm). While the frenar band showed the greatest intra-individual heterogeneity in keratin thickness, there was substantial inter-individual variation seen in all regions. Measurements made by the two laboratories showed high correlation (r = 0.741, 95% CI, 0.533–0.864). We conclude that, despite inter- and intra-individual variability, keratin thickness was similar in the inner and outer foreskin of healthy Ugandan men, and that reduced keratin thickness is not likely to make the inner foreskin more susceptible to HIV acquisition.
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影响因子：
3.7
作者：
Price LB;Liu CM;Johnson KE;Aziz M;Lau MK;Bowers J;Ravel J;Keim PS;Serwadda D;Wawer MJ;Gray RH
通讯作者：
Gray RH
DOI：
10.1097/qad.0b013e328330eda8
发表时间：
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期刊：
AIDS (London, England)
影响因子：
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Gray RH
DOI：
10.1111/j.1600-0897.2010.00933.x
发表时间：
2011-03-01
期刊：
American journal of reproductive immunology (New York, N.Y. : 1989)
影响因子：
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作者：
Ganor, Yonatan;Bomsel, Morgane
通讯作者：
Bomsel, Morgane
DOI：
10.1056/nejmoa0802556
发表时间：
2009-03-26
期刊：
The New England journal of medicine
影响因子：
--
作者：
Tobian AA;Serwadda D;Quinn TC;Kigozi G;Gravitt PE;Laeyendecker O;Charvat B;Ssempijja V;Riedesel M;Oliver AE;Nowak RG;Moulton LH;Chen MZ;Reynolds SJ;Wawer MJ;Gray RH
通讯作者：
Gray RH
影响因子：
3.8
作者：
McCoombe, Scott G.;Short, Roger V.
通讯作者：
Short, Roger V.