Quantitative Measurement of Naïve T Cell Association With Dendritic Cells, FRCs, and Blood Vessels in Lymph Nodes.
Quantitative Measurement of Naïve T Cell Association With Dendritic Cells, FRCs, and Blood Vessels in Lymph Nodes.
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淋巴结中幼稚的T细胞与树突状细胞，FRC和血管的定量测量。
DOI：
10.3389/fimmu.2018.01571
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发表时间：
2018
影响因子：
7.3
通讯作者：
Moses ME
中科院分区：
文献类型：
--
作者：
Tasnim H;Fricke GM;Byrum JR;Sotiris JO;Cannon JL;Moses ME
T细胞在消除致病性感染中起着至关重要的作用。为了激活，naïve T细胞在淋巴结（LNs）中寻找树突状细胞（DCs）。T细胞在LNs中的定位和运动受到包括CCL21在内的趋化因子以及LNs中的多种细胞类型和结构的影响。先前的研究表明，T细胞定位促进DC共定位，导致T:DC相互作用。尽管化学信号、细胞和结构可以对naïve T细胞定位产生影响，但相对较少的研究使用定量方法直接比较T细胞与关键细胞类型的相互作用。在这里，我们使用Pearson相关系数（PCC）和标准化互信息（NMI）来量化naïve T细胞在空间上与dc、成纤维网状细胞（FRCs）和LNs血管的关联程度。我们测量了生理相关区域的空间关联。我们发现T细胞在空间上与FRCs的关联大于其最终靶点dc。我们还研究了一种关键的运动趋化因子受体CCR7在T细胞与dc共定位中的作用。我们发现CCR7缺乏并不会降低naïve T细胞与DC的关联，事实上，与野生型T细胞相比，CCR7−/−T细胞与DC的关联略高。通过揭示这些关联，我们深入了解了驱动T细胞定位的因素，这些因素可能会影响T:DC相互作用和T细胞激活的时间。
T cells play a vital role in eliminating pathogenic infections. To activate, naïve T cells search lymph nodes (LNs) for dendritic cells (DCs). Positioning and movement of T cells in LNs is influenced by chemokines including CCL21 as well as multiple cell types and structures in the LNs. Previous studies have suggested that T cell positioning facilitates DC colocalization leading to T:DC interaction. Despite the influence chemical signals, cells, and structures can have on naïve T cell positioning, relatively few studies have used quantitative measures to directly compare T cell interactions with key cell types. Here, we use Pearson correlation coefficient (PCC) and normalized mutual information (NMI) to quantify the extent to which naïve T cells spatially associate with DCs, fibroblastic reticular cells (FRCs), and blood vessels in LNs. We measure spatial associations in physiologically relevant regions. We find that T cells are more spatially associated with FRCs than with their ultimate targets, DCs. We also investigated the role of a key motility chemokine receptor, CCR7, on T cell colocalization with DCs. We find that CCR7 deficiency does not decrease naïve T cell association with DCs, in fact, CCR7−/− T cells show slightly higher DC association compared with wild type T cells. By revealing these associations, we gain insights into factors that drive T cell localization, potentially affecting the timing of productive T:DC interactions and T cell activation.
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DOI：
10.1084/jem.192.10.1425
发表时间：
2000-11-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gretz JE;Norbury CC;Anderson AO;Proudfoot AE;Shaw S
通讯作者：
Shaw S
影响因子：
3.4
作者：
Fletcher, Patrick A.;Scriven, David R. L.;Moore, Edwin D. W.
通讯作者：
Moore, Edwin D. W.
影响因子：
32.4
作者：
Groom JR;Richmond J;Murooka TT;Sorensen EW;Sung JH;Bankert K;von Andrian UH;Moon JJ;Mempel TR;Luster AD
通讯作者：
Luster AD
DOI：
10.1084/jem.193.9.1105
发表时间：
2001-05-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Baekkevold ES;Yamanaka T;Palframan RT;Carlsen HS;Reinholt FP;von Andrian UH;Brandtzaeg P;Haraldsen G
通讯作者：
Haraldsen G
影响因子：
4.4
作者：
Katakai, T;Hara, T;Shimizu, A
通讯作者：
Shimizu, A