Roles of Aquaporin-3 Water Channels in Volume-Regulatory Water Flow in a Human Epithelial Cell Line
Roles of Aquaporin-3 Water Channels in Volume-Regulatory Water Flow in a Human Epithelial Cell Line
复制标题
Aquaporin-3 水通道在人上皮细胞系体积调节水流中的作用
DOI：
--
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发表时间：
2005
影响因子：
2.4
通讯作者：
Shigeru Morishima
中科院分区：
文献类型：
--
作者：
H. Kida;T. Miyoshi;Ken;Nobuyuki Takahashi;T. Konno;Shunji Ueda;T. Chiba;T. Shimizu;Yasunobu Okada;Shigeru Morishima
膜水转运不仅是渗透压细胞体积变化的重要事件，也是随后的细胞体积调节过程中的重要事件。在此，我们研究了人肠上皮细胞407细胞渗透性肿胀后调节性体积减少(RVD)所涉及的水的运输途径。核磁共振在等渗条件下测得的扩散水渗透系数(PD)远小于渗透梯度下测得的渗透水渗透系数(Pf)。Pf随温度的变化表现出较低的阿累尼乌斯活化能(Ea)(1.6千卡/摩尔)。这些结果表明，渗透水运输过程中存在促进扩散机制。汞离子通道阻滞剂(HgCl2)可降低Pf值。一种非汞的巯基试剂(MMTS)也有效。这些水通道阻滞剂可抑制RVD。RT-PCR和免疫细胞化学显示AQP3水通道在该细胞系中的优势表达。经反义寡核苷酸处理后，AQP3表达下调可抑制RVD反应。因此，AQP3水通道是人上皮细胞容量调节性水运输的重要途径。
Membrane water transport is an essential event not only in the osmotic cell volume change but also in the subsequent cell volume regulation. Here we investigated the route of water transport involved in the regulatory volume decrease (RVD) that occurs after osmotic swelling in human epithelial Intestine 407 cells. The diffusion water permeability coefficient (Pd) measured by NMR under isotonic conditions was much smaller than the osmotic water permeability coefficient (Pf) measured under an osmotic gradient. Temperature dependence of Pf showed the Arrhenius activation energy (Ea) of a low value (1.6 kcal/mol). These results indicate an involvement of a facilitated diffusion mechanism in osmotic water transport. A mercurial water channel blocker (HgCl2) diminished the Pf value. A non-mercurial sulfhydryl reagent (MMTS) was also effective. These blockers of water channels suppressed the RVD. RT-PCR and immunocytochemistry demonstrated predominant expression of AQP3 water channel in this cell line. Downregulation of AQP3 expression induced by treatment with antisense oligodeoxynucleotides was found to suppress the RVD response. Thus, it is concluded that AQP3 water channels serve as an essential pathway for volume-regulatory water transport in, human epithelial cells.
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影响因子：
56.9
作者：
HASEGAWA, H;SKACH, W;VERKMAN, AS
通讯作者：
VERKMAN, AS
DOI：
10.1073/pnas.91.23.10997
发表时间：
1994-11-08
影响因子：
11.1
作者：
ECHEVARRIA, M;WINDHAGER, EE;FRINDT, G
通讯作者：
FRINDT, G
DOI：
10.1073/pnas.86.21.8397
发表时间：
1989-11-01
影响因子：
11.1
作者：
FISCHBARG, J;KUANG, K;LOIKE, J
通讯作者：
LOIKE, J
葡萄糖和油酸吸收过程中大鼠肠道淋巴渗透压。
DOI：
10.1152/ajpgi.1989.257.3.g438
发表时间：
1989
期刊：
The American journal of physiology
影响因子：
--
作者：
Bohlen,HG;Unthank,JL
通讯作者：
Unthank,JL
DOI：
--
发表时间：
1994-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Ma;A. Frigeri;H. Hasegawa;A. Verkman
通讯作者：
T. Ma;A. Frigeri;H. Hasegawa;A. Verkman