Screen the Effective Components of Lycopodii herba on Rheumatoid Arthritis with the Aid of Spectrum-Effect Relationship and Uncover its Potential Mechanism
Screen the Effective Components of Lycopodii herba on Rheumatoid Arthritis with the Aid of Spectrum-Effect Relationship and Uncover its Potential Mechanism
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借助谱效关系筛选石松治疗类风湿性关节炎的有效成分并揭示其潜在机制
DOI：
10.1007/s10753-020-01276-z
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发表时间：
2020-09
期刊：
影响因子：
5.1
通讯作者：
Zhang Yanjun
中科院分区：
文献类型：
--
作者：
Yang Zhen;Yin Qingsheng;Ma Jing;Yang Changshuo;Sheng Yuanyuan;Song Lili;Pang Tan;Zhuang Pengwei;Guo Hong;Zhang Yanjun
伸筋草是一种传统中药，具有祛风除湿（类风湿性关节炎的中医治疗原则和方法）、舒筋活络的功效。但其主要有效成分及其作用机制尚不清楚。在这项研究中，通过用不同浓度的乙醇提取制备了具有略微不同组成的不同SJC样品。然后，评价不同SJC样品对类风湿关节炎（RA）的治疗作用。最后，通过UPLC-Q-TOF/MS指纹图谱与RA作用的谱效关系，筛选有效成分。采用Western blotting方法研究其可能的作用机制。与模型组相比，各参骨胶囊样品给药后，大鼠足爪体积、RF、TNF-α、IL-1β水平均降低。组织病理学结果也证实了SJC可以缓解RA的症状。结合血浆成分的鉴定，认为石松素C、脱N-甲基-α-乌藨子碱、8β-乙酰氧基-12 β-羟基石松素或8β-乙酰氧基-11 α-羟基石松素或8β-羟基-11 α-乙酰氧基石松素是其主要有效成分。其机制可能与AChE/NF-κB信号通路有关。本研究为中药有效成分的筛选提供了一种通用的方法，也为深入了解SJC治疗类风湿关节炎的作用机制提供了理论依据。
Lycopodii herba (SJC), a traditional Chinese medicine, has the effect of dispelling wind and eliminating dampness (a therapeutic principle and method of traditional Chinese medicine for rheumatoid arthritis), relaxing tendon and activating collaterals. However, the major effective components and its therapeutic mechanism were unclear. In this study, different SJC samples with slightly different compositions were prepared by extracting with different concentrations of ethanol. Then, the therapeutic effects on rheumatoid arthritis (RA) of different SJC samples were evaluated. Finally, the spectrum-effect relationship between UPLC-Q-TOF/MS fingerprints and the effect of RA was explored to screen the effective components. Western blotting was used to study the potential mechanism. The volume of hind paw and the level of RF, TNF-α, and IL-1β were lower after administrating with different SJC samples, compared with the model group. Histopathological findings also confirmed that SJC could relieve the symptoms of RA. Combined with identification of the components in plasm from SJC, lycojaponicumin C, des-N-methyl-α-obscurine, 8β-acetoxy-12β-hydroxy-lycopodine or 8β-acetoxy-11α-hydroxy-lycopodine or 8β-hydroxy-11α-acetoxylycopodine were considered to be the major effective components. The mechanism may be related to AChE/NF-κB signaling pathway. This work provides a general method to screen the potential effective components of herb medicines and would be benefit to understand the mechanism of SJC for the treatment of RA.
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影响因子：
4.3
作者：
Mandik-Nayak L;DuHadaway JB;Mulgrew J;Pigott E;Manley K;Sedano S;Prendergast GC;Laury-Kleintop LD
通讯作者：
Laury-Kleintop LD
影响因子：
7.3
作者：
Cooles FAH;Anderson AE;Skelton A;Pratt AG;Kurowska-Stolarska MS;McInnes I;Hilkens CMU;Isaacs JD
通讯作者：
Isaacs JD
影响因子：
1.7
作者：
K. Hanif;M. Kumar;Neetu Singh;R. Shukla
通讯作者：
K. Hanif;M. Kumar;Neetu Singh;R. Shukla
DOI：
10.1002/art.39971
发表时间：
2017-03
期刊：
Arthritis & rheumatology (Hoboken, N.J.)
影响因子：
--
作者：
Zamora-Legoff JA;Krause ML;Crowson CS;Ryu JH;Matteson EL
通讯作者：
Matteson EL
基于谱-毒性关系的UHPLC-QTOF/MS筛选吴茱萸肝毒性成分
影响因子：
4.6
作者：
Liang, Jian;Chen, Yang;Yuan, Jinbin
通讯作者：
Yuan, Jinbin