Hepatocellular Carcinoma Cells Deteriorate the Biophysical Properties of Dendritic Cells
Hepatocellular Carcinoma Cells Deteriorate the Biophysical Properties of Dendritic Cells
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肝细胞癌细胞会破坏树突状细胞的生物物理特性
DOI：
10.1007/s12013-009-9055-6
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发表时间：
2009-07
影响因子：
2.6
通讯作者：
Wen, Zongyao
中科院分区：
文献类型：
--
作者：
Yao, Weijuan;Xu, Guoqiang;Long, Jinhua;Xu, Xiaofeng;Chien, Shu;Wang, Xianwei;Zeng, Zhu;Wen, Zongyao
树突状细胞（Dendritic cells，DC）是一种有效的抗原提呈细胞，可诱导机体产生抗原特异性免疫反应。荷瘤宿主中存在DC功能障碍。为了从多学科角度阐明肿瘤微环境对DC功能的影响，我们研究了与肝癌细胞（HCC）共培养的DC的生物物理特性。结果显示，与正常情况相比，HCC严重损害了未成熟和成熟DC的生物物理特性，包括渗透脆性增加、细胞膜流动性降低、膜粘弹性增加、功能障碍和细胞骨架蛋白F-actin表达增加，以及跨内皮迁移能力下降。DCs的生物物理特性受损可能是肿瘤免疫逃逸机制的许多方面之一。这些结果对于如何提高基于DC的抗肿瘤治疗的效率具有临床和预防意义。
Dendritic cells (DCs) are potent antigen-presenting cells and induce antigen-specific immune responses in the organism. The dysfunction of DCs has been implicated in tumor-bearing host. In order to elucidate the effects of tumor microenvironment on the functions of DCs from interdisciplinary aspects, we characterized the biophysical properties of DCs co-cultured with hepatocellular carcinoma cells (HCC). The results showed that the biophysical characteristics of immature and mature DCs were severely impaired by HCC compared with those under normal conditions, including the increased osmotic fragilities, decreased cell membrane fluidities, increased membrane viscoelastic properties, dysfunction and increased expression of cytoskeleton protein F-actin, as well as the deteriorated transendothelium migration. The impaired biophysical properties of DCs may be one of many aspects of the immune escape mechanisms of tumors. These results are clinically and instructionally significant with regard to how to enhance efficiency of the anti-tumor therapy based on DCs.
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DOI：
10.1096/fj.00-0508hyp
发表时间：
2001-04
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
M. Thurnher;C. Zelle-Rieser;R. Ramoner;G. Bartsch;L. Höltl
通讯作者：
M. Thurnher;C. Zelle-Rieser;R. Ramoner;G. Bartsch;L. Höltl
DOI：
10.1016/s0022-5347(05)68628-4
发表时间：
1999-08
期刊：
The Journal of urology
影响因子：
--
作者：
T. Schwaab;A. Schned;J. Heaney;B. Cole;J. Atzpodien;F. Wittke;M. Ernstoff
通讯作者：
T. Schwaab;A. Schned;J. Heaney;B. Cole;J. Atzpodien;F. Wittke;M. Ernstoff
影响因子：
4.4
作者：
Tourkova, IL;Yurkovetsky, ZR;Shurin, GV
通讯作者：
Shurin, GV
影响因子：
2.6
作者：
Zeng, Z;Liu, X;Chien, S
通讯作者：
Chien, S
影响因子：
3.3
作者：
V. Makarenkova;G. Shurin;I. Tourkova;L. Balkir;G. Pirtskhalaishvili;L. Perez;V. Gerein;J. Siegfried;M. Shurin
通讯作者：
V. Makarenkova;G. Shurin;I. Tourkova;L. Balkir;G. Pirtskhalaishvili;L. Perez;V. Gerein;J. Siegfried;M. Shurin