Structural principles of CRISPR-Cas enzymes used in nucleic acid detection.
Structural principles of CRISPR-Cas enzymes used in nucleic acid detection.
复制标题
用于核酸检测的CRISPR-Cas酶的结构原理。
DOI：
10.1016/j.jsb.2022.107838
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发表时间：
2022-03
影响因子：
3
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Das A;Goswami HN;Whyms CT;Sridhara S;Li H
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-based technology has revolutionized the field of biomedicine with broad applications in genome editing, therapeutics and diagnostics. While a majority of applications involve the RNA-guided site-specific DNA or RNA cleavage by CRISPR enzymes, recent successes in nucleic acid detection rely on their collateral and non-specific cleavage activated by viral DNA or RNA. Ranging in enzyme composition, the mechanism for distinguishing self- from foreign-nucleic acids, the usage of second messengers, and enzymology, the CRISPR enzymes provide a diverse set of diagnosis tools in further innovations. Structural biology plays an important role in elucidating the mechanisms of these CRISPR enzymes. Here we summarize and compare structures of three types of CRISPR enzymes used in nucleic acid detection captured in their respective functional forms and illustrate the current understanding of their activation mechanism.
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DOI：
10.1126/science.aaf5573
发表时间：
2016-08-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Abudayyeh OO;Gootenberg JS;Konermann S;Joung J;Slaymaker IM;Cox DB;Shmakov S;Makarova KS;Semenova E;Minakhin L;Severinov K;Regev A;Lander ES;Koonin EV;Zhang F
通讯作者：
Zhang F
CRISPR-C2C2的两种不同的RNase活性启用了指导RNA处理和RNA检测。
影响因子：
64.8
作者：
East-Seletsky A;O'Connell MR;Knight SC;Burstein D;Cate JH;Tjian R;Doudna JA
通讯作者：
Doudna JA
CRISPR-Cas12a辅助核酸检测
影响因子：
33.5
作者：
Li SY;Cheng QX;Wang JM;Li XY;Zhang ZL;Gao S;Cao RB;Zhao GP;Wang J
通讯作者：
Wang J
影响因子：
4.7
作者：
Li, Linxian;Li, Shiyuan;Wang, Jin
通讯作者：
Wang, Jin
影响因子：
16.8
作者：
Knott GJ;East-Seletsky A;Cofsky JC;Holton JM;Charles E;O'Connell MR;Doudna JA
通讯作者：
Doudna JA