The anisin1 gene encodes a defensin-like protein and supports the fitness of Aspergillus nidulans.
The anisin1 gene encodes a defensin-like protein and supports the fitness of Aspergillus nidulans.
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DOI：
10.1007/s00203-011-0773-y
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发表时间：
2012-06
影响因子：
2.8
通讯作者：
Marx, Florentine
中科院分区：
文献类型：
--
作者：
Eigentler, Andrea;Pocsi, Istvan;Marx, Florentine
在构巢曲霉基因组中，一种防御素样蛋白Anisin 1与蚊子防御素AaDefA 1具有高度同源性。到目前为止，还没有关于丝状子囊菌防御素的研究。因此，我们对A.与参考菌株相比，该缺失突变体遭受有丝分裂孢子发育缺陷，并且在42°C下产生较少的分生孢子。在A.在nidulans野生型中，茴香素1的表达与无性发育的中心调节因子brlA和H2 O2胁迫的主要清除剂catB相关，catB指示发育中的真菌中的细胞分化。有趣的是，brlA和茴香素1的表达在ΔsrrA菌株中被解除调节，该菌株在组氨酸-天冬氨酸（His-Asp）磷酸化信号通路中起核心作用，并显示出无性发育和H2 O2解毒受损。在A.在His-Asp磷酸传递信号途径的野生型和其他突变体中，茴香素1被抑制，但在ΔsrrA背景下被解除抑制，并且茴香素1的转录在该突变体中通过H2 O2的加入进一步增加。因此，我们得出结论，分泌蛋白茴香素1有助于优化发展的A。nidulans，我们进一步提出，它有一个传感/信号功能的H2 O2水平升高。本文的在线版本（doi：10.1007/s 00203 -011-0773-y）包含补充材料，可供授权用户使用。
In the genome of Aspergillus nidulans, a defensin-like protein, Anisin1, was annotated with high homology to the mosquito defensin AaDefA1. So far, no studies exist on defensins from filamentous ascomycetes. Therefore, we characterized the anisin1 gene in A. nidulans and generated a deletion mutant, which suffered from a defect in mitospore development and produced less conidia at 42°C compared to the reference strain. In surface cultures of A. nidulans wild type, the anisin1 expression correlated with that of the central regulator for asexual development, brlA, and with the major scavanger of H2O2 stress, catB, which is indicative for cell differentiation in developing fungi. Interestingly, brlA and anisin1 expressions were deregulated in a ΔsrrA strain that covers a central role in the histidine-to-aspartate (His-Asp) phosphorelay signaling pathway and shows impaired asexual development and H2O2 detoxification. In submers cultures of A. nidulans wild type and other mutants of the His-Asp phosphorelay signaling pathway, anisin1 was repressed, but derepressed in a ΔsrrA background, and anisin1 transcription was further increased in this mutant by H2O2 addition. We therefore conclude that the secreted protein Anisin1 contributes to the optimal development of A. nidulans and we further propose that it has a sensing/signaling function for elevated H2O2 levels. The online version of this article (doi:10.1007/s00203-011-0773-y) contains supplementary material, which is available to authorized users.
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影响因子：
3.1
作者：
Hegedues, Nikoletta;Sigl, Claudia;Zadra, Ivo;Pocsi, Istvan;Marx, Florentine
通讯作者：
Marx, Florentine
影响因子：
3.6
作者：
KULMBURG, P;MATHIEU, M;FELENBOK, B
通讯作者：
FELENBOK, B
DOI：
10.1111/j.1742-4658.2009.07011.x
发表时间：
2009-05
期刊：
The FEBS journal
影响因子：
--
作者：
Batta G;Barna T;Gáspári Z;Sándor S;Kövér KE;Binder U;Sarg B;Kaiserer L;Chhillar AK;Eigentler A;Leiter E;Hegedüs N;Pócsi I;Lindner H;Marx F
通讯作者：
Marx F
DOI：
10.2174/1568005024605855
发表时间：
2002-03-01
期刊：
Current Drug Targets - Infectious Disorders
影响因子：
--
作者：
Hancock, R. E. W.;Patrzykat, A.
通讯作者：
Patrzykat, A.
影响因子：
1.6
作者：
Hagiwara, Daisuke;Matsubayashi, Yoshihiro;Mizuno, Takeshi
通讯作者：
Mizuno, Takeshi