Evaluation of Saliva as a Matrix for RT-PCR Analysis and Two Rapid Antigen Tests for the Detection of SARS-CoV-2.
Evaluation of Saliva as a Matrix for RT-PCR Analysis and Two Rapid Antigen Tests for the Detection of SARS-CoV-2.
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DOI：
10.3390/v14091931
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发表时间：
2022-08-30
期刊：
影响因子：
--
通讯作者：
Vandamme S
中科院分区：
文献类型：
--
作者：
De Meyer J;Goris H;Mortelé O;Spiessens A;Hans G;Jansens H;Goossens H;Matheeussen V;Vandamme S
使用唾液检测严重急性呼吸综合征冠状病毒2(SARS-CoV-2)引发了争论，因为假定敏感性较低，且缺乏标准化。我们的目的是评估(I)ORACollectTM设备收集的唾液作为SARS-CoV-2逆转录聚合酶链式反应(RT-PCR)基质的性能特征，(Ii)2唾液快速抗原检测(AgRDT)。对342例门诊患者同时采集鼻咽拭子和唾液标本进行SARS-CoV-2 RT-PCR检测。此外，54和123还进行了V-ChekTM或WvislingTM唾液AgRDT。总体而言，35%的人通过鼻咽拭子筛查出SARS-CoV-2阳性。唾液作为RT-PCR的基质，其特异度为96.5%，阴性预测值为91.3%。有趣的是，在8名被认为是唾液假阳性的患者中，有6名在2至9天后通过鼻咽采样重新检测为阳性。V-ChekTM和WvislingTM AgRDT均缺乏敏感性，NPV分别为66.9%和67.3%。RT-PCR证实唾液是检测SARS-CoV-2的灵敏而特异的基质。在这种情况下，唾液可能比鼻咽拭子具有更早的检测窗口。相比之下，两种AgRDT显示出令人无法接受的低敏感性和NPV。
The use of saliva for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sparks debate due to presumed lower sensitivity and lack of standardization. Our aim was to evaluate the performance characteristics of (i) saliva collected by the ORAcollectTM device as a matrix for SARS-CoV-2 reverse-transcriptase polymerase chain reaction (RT-PCR), and (ii) 2 saliva rapid antigen tests (AgRDT). From 342 ambulatory individuals, both a nasopharyngeal swab and saliva sample via ORAcollectTM were obtained for a SARS-CoV-2 RT-PCR test. Furthermore, 54 and 123 additionally performed the V-ChekTM or WhistlingTM saliva AgRDT. In total, 35% of individuals screened positive for SARS-CoV-2 via nasopharyngeal swab. Saliva, as a matrix for the RT-PCR, had a specificity of 96.5% and a negative predictive value (NPV) of 91.3%. Interestingly, 6 out of 8 patients thought to be false positive in saliva re-tested positive by nasopharyngeal sampling after 2 to 9 days. Both V-ChekTM and WhistlingTM AgRDT had a lack of sensitivity, resulting in an NPV of 66.9 and 67.3%, respectively. Saliva proved to be a sensitive and specific matrix for SARS-CoV-2 detection by the RT-PCR. In this setting, saliva might have an earlier window of detection than the nasopharyngeal swab. By contrast, both AgRDT showed an unacceptably low sensitivity and NPV.
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DOI：
10.3390/diagnostics12010110
发表时间：
2022-01-04
期刊：
Diagnostics (Basel, Switzerland)
影响因子：
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作者：
Khalid MF;Selvam K;Jeffry AJN;Salmi MF;Najib MA;Norhayati MN;Aziah I
通讯作者：
Aziah I
IGA主导对SARS-COV-2的早期中和抗体反应。
影响因子：
17.1
作者：
Sterlin D;Mathian A;Miyara M;Mohr A;Anna F;Claër L;Quentric P;Fadlallah J;Devilliers H;Ghillani P;Gunn C;Hockett R;Mudumba S;Guihot A;Luyt CE;Mayaux J;Beurton A;Fourati S;Bruel T;Schwartz O;Lacorte JM;Yssel H;Parizot C;Dorgham K;Charneau P;Amoura Z;Gorochov G
通讯作者：
Gorochov G
影响因子：
14.2
作者：
Pasomsub, E.;Watcharananan, S. P.;Phuphuakrat, A.
通讯作者：
Phuphuakrat, A.
影响因子：
4.5
作者：
Caruana G;Croxatto A;Kampouri E;Kritikos A;Opota O;Foerster M;Brouillet R;Senn L;Lienhard R;Egli A;Pantaleo G;Carron PN;Greub G
通讯作者：
Greub G
DOI：
10.1016/j.ijid.2022.03.037
发表时间：
2022-06
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
--
作者：
Jegerlehner S;Suter-Riniker F;Jent P;Bittel P;Nagler M
通讯作者：
Nagler M