Chronic neuromuscular electrical stimulation of paralyzed hindlimbs in a rodent model.
Chronic neuromuscular electrical stimulation of paralyzed hindlimbs in a rodent model.
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DOI：
10.1016/j.jneumeth.2009.06.043
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发表时间：
2009-10-15
影响因子：
3
通讯作者：
Abbas, James J.
中科院分区：
文献类型：
--
作者：
Jung, Ranu;Ichihara, Kazuhiko;Venkatasubramanian, Ganapriya;Abbas, James J.
关键词：
神经肌肉电刺激（NMES）可用于激活瘫痪或麻痹的肌肉，以产生功能性或治疗性运动。本研究的目的是建立脊髓损伤（SCI）后nmes辅助运动治疗的啮齿动物模型，从而从分子到系统水平研究nmes诱导可塑性的机制。该模型的开发需要电极和肌肉刺激部位的精确映射，选择性激活肌肉以产生足够强度的分级收缩的能力，植入电极在肌肉内的稳定锚定以及在治疗窗口数周内功能可靠的稳定表现。将设计的电极长期植入脊髓横断大鼠后肢肌肉。通过描述植入后6-8周的强度持续曲线、等距扭矩恢复曲线和关节角度偏移运动学，评估电极的机械和电气稳定性以及实现适当肌肉恢复和关节角度偏移的能力。结果表明，定制设计的电极和植入技术在没有每日NMES（8周）和植入后3周（6周）开始每日NMES的情况下都能提供足够的锚定并产生稳定可靠的肌肉再生。完成的工作建立了一个啮齿类动物模型，可用于研究脊髓损伤后基于nmes的运动治疗的神经可塑性机制，并优化其交付时间。
Neuromuscular electrical stimulation (NMES) can be used to activate paralyzed or paretic muscles to generate functional or therapeutic movements. The goal of this research was to develop a rodent model of NMES-assisted movement therapy after spinal cord injury (SCI) that will enable investigation of mechanisms of NMES-induced plasticity, from the molecular to systems level. Development of the model requires accurate mapping of electrode and muscle stimulation sites, the capability to selectively activate muscles to produce graded contractions of sufficient strength, stable anchoring of the implanted electrode within the muscles and stable performance with functional reliability over several weeks of the therapy window. Custom designed electrodes were implanted chronically in hindlimb muscles of spinal cord transected rats. Mechanical and electrical stability of electrodes and the ability to achieve appropriate muscle recruitment and joint angle excursion were assessed by characterizing the strength duration curves, isometric torque recruitment curves and kinematics of joint angle excursion over 6–8 weeks post implantation. Results indicate that the custom designed electrodes and implantation techniques provided sufficient anchoring and produced stable and reliable recruitment of muscles both in the absence of daily NMES (for 8 weeks) as well as with daily NMES that is initiated 3 weeks post implantation (for 6 weeks). The completed work establishes a rodent model that can be used to investigate mechanisms of neuroplasticity that underlie NMES-based movement therapy after spinal cord injury and to optimize the timing of its delivery.
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DOI：
10.1109/tnsre.2003.823268
发表时间：
2004-03-01
影响因子：
4.9
作者：
Aoyagi, Y;Mushahwar, VK;Prochazka, A
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Jung, Ranu
DOI：
10.1109/tbme.2008.2008193
发表时间：
2009-02
期刊：
IEEE transactions on bio-medical engineering
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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