Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues
Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues
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时间分辨荧光免疫分析法定量测定可食用动物组织中的泰乐菌素和替米考星
DOI：
10.1007/s11434-013-5749-7
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发表时间：
2013-03
影响因子：
--
通讯作者：
Luo JinHua
中科院分区：
文献类型：
--
作者：
Xu Jian;He HongQiu;Niu XiaoDong;Luo JinHua
To quantitatively determine tylosin and tilmicosin in edible animal tissues, a time-resolved fluoroimmunoassay (TRFIA) has been developed and validated. For this purpose, desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates, standards and samples were loaded and, finally, diluted europium-labeled anti-tylosin antibodies were added. Results show that the limit of detection for tylosin was 0.03 ng mL−1and that for tilmicosin was 0.05 ng mL−1. The recoveries were 73.6% to 120.5%, with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50–200 ng g−1. There was good correlation (R2>0.99) between the TRFIA, an enzyme-linked immunosorbent assay and high performance liquid chromatography data. In conclusion, the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening. The method is expected to be widely applicable.
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影响因子：
6.2
作者：
C. Situ;C. Elliott
通讯作者：
C. Situ;C. Elliott
影响因子：
1.6
作者：
M. Juhel-Gaugain;B. Anger;M. Laurentie
通讯作者：
M. Juhel-Gaugain;B. Anger;M. Laurentie
影响因子：
--
作者：
Zhen Zhang;Jing-fu Liu;Yan Yao;G. Jiang
通讯作者：
Zhen Zhang;Jing-fu Liu;Yan Yao;G. Jiang
影响因子：
4.1
作者：
Jue Zhang;Lei Gao;Bin Zhou;Lan Zhu;Yi Zhang;Biao Huang
通讯作者：
Jue Zhang;Lei Gao;Bin Zhou;Lan Zhu;Yi Zhang;Biao Huang
DOI：
10.1080/02652030601075470
发表时间：
2007-03
期刊：
Food Additives & Contaminants
影响因子：
--
作者：
U. Vincent;G. Gizzi;C. Holst;J. D. Jong;J. Michard
通讯作者：
U. Vincent;G. Gizzi;C. Holst;J. D. Jong;J. Michard