Nucleosome depletion alters the chromatin structure of Saccharomyces cerevisiae centromeres
Nucleosome depletion alters the chromatin structure of Saccharomyces cerevisiae centromeres
复制标题
核小体耗尽改变酿酒酵母着丝粒的染色质结构
DOI：
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发表时间：
1990
影响因子：
5.3
通讯作者：
K. Bloom
中科院分区：
文献类型：
--
作者：
M. Saunders;E. Yeh;M. Grunstein;K. Bloom
酿酒酵母着丝粒DNA被包装到约200个碱基对的DNA的高度耐核酸酶的染色质核心中。染色体III中的着丝粒的结构比160个碱基对的核小体核心稍大，并包含保守的着丝粒DNA元件（CDE I，II和III）。广泛的突变分析揭示了着丝粒功能的序列要求。影响着丝粒分离特性的突变在体内也表现出染色质结构的改变。因此，由核酸酶消化描绘的结构与功能性着丝粒相关。我们已经确定了组蛋白对这种独特结构组织的贡献。通过组蛋白H2B或H4的抑制而导致的核小体缺失使细胞不能进行染色体分离。组蛋白阻遏导致着丝粒DNA的核酸酶敏感性增加，其中高达40%的CEN3 DNA分子变得可用于核酸裂解攻击。核小体耗竭也导致CEN3周围DNA中核酸酶切割位点分布的改变。这些数据提供了第一个迹象表明，真正的核小体亚基侧翼的着丝粒，并建议核小体可能是中央核心的着丝粒本身。
Saccharomyces cerevisiae centromeric DNA is packaged into a highly nuclease-resistant chromatin core of approximately 200 base pairs of DNA. The structure of the centromere in chromosome III is somewhat larger than a 160-base-pair nucleosomal core and encompasses the conserved centromere DNA elements (CDE I, II, and III). Extensive mutational analysis has revealed the sequence requirements for centromere function. Mutations affecting the segregation properties of centromeres also exhibit altered chromatin structures in vivo. Thus the structure, as delineated by nuclease digestion, correlated with functional centromeres. We have determined the contribution of histone proteins to this unique structural organization. Nucleosome depletion by repression of either histone H2B or H4 rendered the cell incapable of chromosome segregation. Histone repression resulted in increased nuclease sensitivity of centromere DNA, with up to 40% of CEN3 DNA molecules becoming accessible to nucleolytic attack. Nucleosome depletion also resulted in an alteration in the distribution of nuclease cutting sites in the DNA surrounding CEN3. These data provide the first indication that authentic nucleosomal subunits flank the centromere and suggest that nucleosomes may be the central core of the centromere itself.
酿酒酵母 VI 号染色体着丝粒 DNA 的突变分析。
影响因子：
5.3
作者：
Hegemann,JH;Shero,JH;Cottarel,G;Philippsen,P;Hieter,P
通讯作者：
Hieter,P
影响因子：
3.3
作者：
Susan Cumberledge;J. Carbon
通讯作者：
Susan Cumberledge;J. Carbon
改变的酵母着丝粒的染色质结构。
DOI：
10.1073/pnas.85.1.175
发表时间：
1988
影响因子：
11.1
作者：
Saunders,M;Fitzgerald-Hayes,M;Bloom,K
通讯作者：
Bloom,K