A transposon insertion in FLOWERING LOCUS T is associated with delayed flowering in Brassica rapa.
A transposon insertion in FLOWERING LOCUS T is associated with delayed flowering in Brassica rapa.
复制标题
FLOWERING LOCUS T 中的转座子插入与白菜延迟开花有关。
DOI：
10.1016/j.plantsci.2015.10.007
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发表时间：
2015-12
期刊：
影响因子：
5.2
通讯作者：
Wu, Jian
中科院分区：
文献类型：
--
作者：
Liang, Jianli;Aarts, M.G.Mark;Wang, Xiaowu;Wu, Jian
漫长的白昼和春化加速了油菜从营养生长到生殖生长的转变。在植物到达收获阶段之前抽苔是一个严重的问题。蔬菜作物种植。开花时间的遗传解剖对早熟抗栓苗育种具有重要意义。rapacrops。利用重组自交系（RIL）群体，分别在A02和A07染色体上检测到两个主要的开花时间数量性状位点（qtl）。我们假设拟南芥开花位点T（FT）基因的同源基因asBrFT2是定位于A07的QTL的候选基因。在其中一个亲本系中检测到brft2的第二个内含子中的转座子插入，预计这将产生一个功能丧失等位基因。转录分析显示，brft2转录本不存在于携带突变等位基因的亲本系中。与携带野生型brft2等位基因的ril相比，仅携带突变brft2等位基因的ril无论生长季节如何都延迟开花。这些数据表明brft2参与了花期调控，控制了b的花期。拉伯。
Long days and vernalization accelerate the transition from vegetative growth to reproductive growth inBrassica rapa. Bolting before plants reach the harvesting stage is a serious problem inB. rapavegetable crop cultivation. The genetic dissection of flowering time is important for breeding of premature bolting-resistantB. rapacrops. Using a recombinant inbred line (RIL) population, we twice detected two major quantitative trait loci (QTLs) for flowering time in two different growing seasons that were located on chromosomes A02 and A07, respectively. We hypothesized that an orthologue of theArabidopsis thaliana FLOWERING LOCUS T(FT) gene, named asBrFT2, was the candidate gene underlying the QTL localized to A07. A transposon insertion in the second intron ofBrFT2was detected in one of the parental lines, which was predicted to generate a loss-of-function allele. Transcription analysis revealed that theBrFT2transcript was not present in the parental line that harbored the mutated allele. RILs carrying only the mutatedBrFT2allele showed delayed flowering regardless of growing seasons when compared to RILs carrying the wild-typeBrFT2allele. These data suggest thatBrFT2is involved in flowering time regulation in controlling flowering time inB. rapa.
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影响因子：
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