A Self-Digitization Dielectrophoretic (SD-DEP) Chip for High-Efficiency Single-Cell Capture, On-Demand Compartmentalization, and Downstream Nucleic Acid Analysis.
A Self-Digitization Dielectrophoretic (SD-DEP) Chip for High-Efficiency Single-Cell Capture, On-Demand Compartmentalization, and Downstream Nucleic Acid Analysis.
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DOI：
10.1002/anie.201807314
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发表时间：
2018-08-27
期刊：
影响因子：
--
通讯作者：
Chiu DT
中科院分区：
文献类型：
--
作者：
Qin Y;Wu L;Schneider T;Yen GS;Wang J;Xu S;Li M;Paguirigan AL;Smith JL;Radich JP;Anand RK;Chiu DT
本文介绍了SD-DEP芯片的设计和制造，该芯片具有用于单细胞操作和下游核酸分析的简单组件。该设备采用传统的DEP和绝缘体DEP来创建局部电场，该电场被定制为大约单个细胞的大小，从而实现高效的单细胞捕获。在集成的微型装置中进行细胞操作、区室化、裂解和分析的多步骤程序，消耗最少的试剂，最大限度地减少污染，减少裂解物稀释，并增加测定灵敏度。这里开发的平台可能是精确医学单细胞研究中一个有前途的强大工具。利用传统介电电泳和绝缘体介电电泳的优点，设计了自数字化介电电泳（SD-DEP）芯片，以产生用于单细胞操作和下游核酸分析的局部电场。细胞操作，裂解和化学分析的多步骤程序中进行的集成的微型装置，消耗最少的试剂，最大限度地减少污染，并增加测定灵敏度。
This communication describes the design and fabrication of SD-DEP chip with simple components for single-cell manipulation and downstream nucleic acid analysis. The device employed the traditional DEP and insulator DEP to create the local electric field that is tailored to approximately the size of single cells, enabling highly efficient single-cell capture. The multistep procedures of cell manipulation, compartmentalization, lysis, and analysis were performed in the integrated microdevice, consuming minimal reagents, minimizing contamination, decreasing lysate dilution, and increasing assay sensitivity. The platform developed here could be a promising and powerful tool in single-cell research for precise medicine. Self-digitization dielectrophoretic (SD-DEP) chip was designed by employing the traditional DEP and insulator DEP to create the local electric field for single-cell manipulation and downstream nucleic acid analysis. The multistep procedures of cell manipulation, lysis, and chemical analysis were performed in the integrated microdevice, consuming minimal reagents, minimizing contamination, and increasing assay sensitivity.
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影响因子：
14.8
作者：
Mazutis, Linas;Gilbert, John;Ung, W. Lloyd;Weitz, David A.;Griffiths, Andrew D.;Heyman, John A.
通讯作者：
Heyman, John A.
影响因子：
3.8
作者：
Altomare, L;Borgatti, M;Gambari, R
通讯作者：
Gambari, R
影响因子：
46.9
作者：
Love, J. Christopher;Ronan, Jehnna L.;Ploegh, Hidde L.
通讯作者：
Ploegh, Hidde L.
DOI：
10.1038/nrg.2017.15
发表时间：
2017-06
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
Prakadan SM;Shalek AK;Weitz DA
通讯作者：
Weitz DA
影响因子：
6.1
作者：
Gansen A;Herrick AM;Dimov IK;Lee LP;Chiu DT
通讯作者：
Chiu DT