Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3.

Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3.
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深海细菌 Dietzia maris As-13-3 的石油降解和生物表面活性剂生产。

DOI:
10.3389/fmicb.2014.00711
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发表时间:
2014
影响因子:
5.2
通讯作者:
Shao Z
Shao Z
中科院分区:
生物学2区
文献类型:
--
作者:
Wang W;Cai B;Shao Z

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最近对极端环境的研究揭示了许多具有生物活性的天然产物。然而,深海极端环境中的生物表面活性剂生产菌株在很大程度上是未知的。本文研究了从深海热液中分离得到的Dietzia maris As-13-3菌株产生双鼠李糖脂作为生物表面活性剂的能力。纯化的二鼠李糖脂的临界胶束浓度(CMC)被测定为120 mgL−1,并且它将水的表面张力从74 ± 0.2 mN m − 1降低到38 ± 0.2 mN m−1。此外,还通过基因组测序分析了烷烃代谢途径相关基因和二鼠李糖脂生物合成相关基因。maris As-13-3和实时荧光定量PCR(Q-PCR)。Q-PCR分析表明,这些基因均受到正十四烷、正十六烷和降植烷的诱导。据我们所知,这是第一次报道的完整途径的双鼠李糖脂的合成过程中,属迪茨。因此,本研究为深入了解迪茨菌在石油降解和生物表面活性剂产生方面的作用提供了依据,并将有助于评价迪茨菌在海洋除油方面的潜力。
Recent investigations of extreme environments have revealed numerous bioactive natural products. However, biosurfactant-producing strains from deep sea extreme environment are largely unknown. Here, we show that Dietzia maris As-13-3 isolated from deep sea hydrothermal field could produce di-rhamnolipid as biosurfactant. The critical micelle concentration (CMC) of the purified di-rhamnolipid was determined to be 120 mgL−1, and it lowered the surface tension of water from 74 ± 0.2 to 38 ± 0.2 mN m−1. Further, the alkane metabolic pathway-related genes and di-rhamnolipid biosynthesis-related genes were also analyzed by the sequencing genome of D. maris As-13-3 and quantitative real-time PCR (Q-PCR), respectively. Q-PCR analysis showed that all these genes were induced by n-Tetradecane, n-Hexadecane, and pristane. To the best of our knowledge, this is first report about the complete pathway of the di-rhamnolipid synthesis process in the genus Dietzia. Thus, our study provided the insights into Dietzia in respects of oil degradation and biosurfactant production, and will help to evaluate the potential of Dietzia in marine oil removal.
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