Gene expression profile of a selection of Polycomb Group genes during zebrafish embryonic and germ line development.
Gene expression profile of a selection of Polycomb Group genes during zebrafish embryonic and germ line development.
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DOI：
10.1371/journal.pone.0200316
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Kamminga LM
中科院分区：
文献类型：
--
作者：
Chrispijn ND;Andralojc KM;Castenmiller C;Kamminga LM
Polycomb Group (PcG) 基因是转录抑制因子，被认为在发育和分化过程中很重要。由于 PcGs 蛋白在干细胞生物学和肿瘤发生中的作用，人们对它们非常感兴趣。在这项研究中，我们表征了斑马鱼成年种系和胚胎发生过程中一系列 PcG 基因的表达。在成人中，选定的 PcG 基因的表达被发现在种系中比体细胞组织中丰富。因此，通过整体原位杂交分析了成年斑马鱼种系中选定的 PcG 基因的表达模式。我们在卵子发生和精子发生的早期阶段检测到了所测试的 PcG 基因转录本的存在。这种配子发生早期阶段的丰富表达也在受精后 4 周和 5 周发育中的性腺中观察到。此外，斑马鱼胚胎还被用来研究发育过程中一系列 PcG 基因的时空表达模式。我们测试的 PcG 基因是母源负载的，并且在早期发育阶段普遍表达，除了 ezh1 之外。所评估的 PcG 基因的表达在前面变得丰富并且在组织规范期间更加明确。这里显示的数据是体内功能性 PcG 基因研究的重要资源。
Polycomb Group (PcG) genes are transcriptional repressors that are described to be important during development and differentiation. There is significant interest in PcGs proteins because of their role in stem cell biology and tumorigenesis. In this study we characterize the expression of a selection of PcG genes in the adult germline of zebrafish and during embryogenesis. In adults, expression of selected PcG genes is found to be enriched in germ line over somatic tissues. Therefore, the germ line of adult zebrafish was analyzed for the expression pattern of a selection of PcG genes by whole mount in situ hybridization. We detected presence of the tested PcG gene transcripts at early stages of both oogenesis and spermatogenesis. This enriched expression for early stages of gametogenesis is also observed in developing gonads at 4 and 5 weeks post fertilization. Additionally, zebrafish embryos were used to study the spatio-temporal expression patterns of a selection of PcG genes during development. The PcG genes that we tested are maternally loaded and ubiquitously expressed at early developmental stages, except of ezh1. The expression of the PcG genes that were assessed becomes enriched anteriorly and is more defined during tissue specification. The data shown here is an important resource for functional PcG gene studies in vivo.
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影响因子：
5.3
作者：
O'Carroll, D;Erhardt, S;Jenuwein, T
通讯作者：
Jenuwein, T
EZH1和EZH2通过不同的机制保持抑制性染色质。
影响因子：
16
作者：
Margueron, Raphael;Li, Guohong;Sarma, Kavitha;Blais, Alexandre;Zavadil, Jiri;Woodcock, Christopher L.;Dyniacht, Brian D.;Reinberg, Danny
通讯作者：
Reinberg, Danny
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
3.7
作者：
Dupret B;Völkel P;Le Bourhis X;Angrand PO
通讯作者：
Angrand PO
影响因子：
9.2
作者：
Bender, LB;Cao, R;Strome, S
通讯作者：
Strome, S