Synthesis and Characterization of Silk Ionomers for Layer-by-Layer Electrostatic Deposition on Individual Mammalian Cells.
Synthesis and Characterization of Silk Ionomers for Layer-by-Layer Electrostatic Deposition on Individual Mammalian Cells.
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DOI：
10.1021/acs.biomac.0c00523
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发表时间：
2020-07-13
影响因子：
6.2
通讯作者：
Kaplan DL
中科院分区：
文献类型：
--
作者：
Hasturk O;Sahoo JK;Kaplan DL
用聚合物层纳米包覆单个哺乳动物细胞在生物技术和生物医学工程应用中越来越受到关注。聚电解质在带负电荷的细胞表面上的静电逐层（LbL）沉积已经用于使用在生理条件下具有净电荷的合成或天然聚合物的细胞纳米涂层。在这里，我们以前的合成丝基离聚物通过修改丝素蛋白（SF）与聚谷氨酸（PG）和聚赖氨酸（PL）被利用的哺乳动物细胞的纳米涂层。SF-PL构建体对哺乳动物细胞具有细胞毒性，因此利用通过再生SF链的羧化和胺化合成丝离聚物的替代方法。通过优化材料性能和孵育缓冲液的组成，丝离聚物可以静电组装在鼠成纤维细胞和人间充质干细胞（hMSCs）的表面上，形成纳米级多层膜，而不会显着损害细胞活力。由此产生的基于丝的蛋白质纳米壳是瞬时的，并随着时间的推移而降解，从而允许细胞增殖。本文提出的策略为基于丝的人工细胞壁内哺乳动物细胞的细胞相容性纳米封装提供了基础，对哺乳动物细胞表面工程的未来研究以及细胞治疗，3D打印和保存的实用性具有潜在的益处。
Nanocoating of individual mammalian cells with polymer layers has been of increasing interest in biotechnology and biomedical engineering applications. Electrostatic layer-by-layer (LbL) deposition of polyelectrolytes on negatively charged cell surfaces has been utilized for cell nanocoatings using synthetic or natural polymers with a net charge at physiological conditions. Here, our previous synthesis of silk-based ionomers through modification of silk fibroin (SF) with polyglutamate (PG) and polylysine (PL) was exploited for the nanocoating of mammalian cells. SF-PL constructs were cytotoxic to mammalian cells, thus an alternative approach for the synthesis of silk ionomers through carboxylation and amination of regenerated SF chains was utilized. Through the optimization of material properties and composition of incubation buffers, silk ionomers could be electrostatically assembled on the surface of murine fibroblasts and human mesenchymal stem cells (hMSCs) to form nanoscale multilayers without significantly impairing cell viability. The resulting silk-based protein nanoshells were transient and degraded over time, allowing for cell proliferation. The strategies presented here provide a basis for the cytocompatible nanoencapsulation of mammalian cells within silk-based artificial cell walls, with potential benefits for future studies on surface engineering of mammalian cells, as well as for utility in cell therapies, 3D printing and preservation.
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