Home life: factors structuring the bacterial diversity found within and between homes.
Home life: factors structuring the bacterial diversity found within and between homes.
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DOI：
10.1371/journal.pone.0064133
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Menninger HL
中科院分区：
文献类型：
--
作者：
Dunn RR;Fierer N;Henley JB;Leff JW;Menninger HL
我们大部分时间都在室内度过，在那里我们接触到生活在我们家的表面和空气中的各种不同的微生物。尽管它们无处不在且丰富，但我们对家庭内发现的微生物多样性以及微生物群落的组成和多样性如何在家庭内不同位置发生变化的了解有限。在这里，我们利用细菌 16S rRNA 基因的高通量测序，检查了美国北卡罗来纳州罗利-达勒姆地区 40 户家庭中每户 9 个不同地点发现的细菌群落的多样性。我们发现，每个采样地点都含有彼此不同的细菌群落，定期清洁的表面通常比不经常清洁的表面具有较低水平的多样性。细菌群落的这些特定位置差异可能与使用模式以及分散到这些位置的细菌可能来源的差异直接相关。最后，我们研究了不同家庭的细菌多样性差异是否可归因于室外环境因素、室内栖息地结构或家庭居住者。我们发现狗的存在对家庭内多个位置的细菌群落组成有显着影响，因为狗所居住的家庭拥有更多样化的群落和与狗相关的细菌类群的相对丰度。此外，我们发现沉积在屋外表面上的细菌类型与屋内发现的细菌类型之间存在显着相关性，这强调了屋外的微生物可以对生活在我们屋内表面上的微生物群落产生直接影响。这项工作首次对家庭中发现的微生物群落以及塑造家庭内部和家庭之间这些群落结构的因素进行了全面分析。
Most of our time is spent indoors where we are exposed to a wide array of different microorganisms living on surfaces and in the air of our homes. Despite their ubiquity and abundance, we have a limited understanding of the microbial diversity found within homes and how the composition and diversity of microbial communities change across different locations within the home. Here we examined the diversity of bacterial communities found in nine distinct locations within each of forty homes in the Raleigh-Durham area of North Carolina, USA, using high-throughput sequencing of the bacterial 16S rRNA gene. We found that each of the sampled locations harbored bacterial communities that were distinct from one another with surfaces that are regularly cleaned typically harboring lower levels of diversity than surfaces that are cleaned infrequently. These location-specific differences in bacterial communities could be directly related to usage patterns and differences in the likely sources of bacteria dispersed onto these locations. Finally, we examined whether the variability across homes in bacterial diversity could be attributed to outdoor environmental factors, indoor habitat structure, or the occupants of the home. We found that the presence of dogs had a significant effect on bacterial community composition in multiple locations within homes as the homes occupied by dogs harbored more diverse communities and higher relative abundances of dog-associated bacterial taxa. Furthermore, we found a significant correlation between the types of bacteria deposited on surfaces outside the home and those found inside the home, highlighting that microbes from outside the home can have a direct effect on the microbial communities living on surfaces within our homes. Together this work provides the first comprehensive analysis of the microbial communities found in the home and the factors that shape the structure of these communities both within and between homes.
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影响因子：
3.7
作者：
Hewitt KM;Gerba CP;Maxwell SL;Kelley ST
通讯作者：
Kelley ST
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.1205624109
发表时间：
2012-05-22
影响因子：
11.1
作者：
Hanski, Ilkka;von Hertzen, Leena;Haahtela, Tari
通讯作者：
Haahtela, Tari
DOI：
10.1073/pnas.1000162107
发表时间：
2010-04-06
影响因子：
11.1
作者：
Fierer, Noah;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
5.1
作者：
Flores, Gilberto E.;Bates, Scott T.;Fierer, Noah
通讯作者：
Fierer, Noah