Office space bacterial abundance and diversity in three metropolitan areas.
Office space bacterial abundance and diversity in three metropolitan areas.
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DOI：
10.1371/journal.pone.0037849
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kelley ST
中科院分区：
文献类型：
--
作者：
Hewitt KM;Gerba CP;Maxwell SL;Kelley ST
发达国家的人们大约90%的时间都在室内度过，但我们对建筑环境中微生物的来源和多样性知之甚少。在这项研究中，我们结合了基于培养的细胞计数和环境核糖体RNA（rRNA）基因序列的多重焦磷酸测序，以调查办公空间的细菌多样性在三个大都市地区。在每个城市的30个不同办公室（90个办公室，共450个样本），使用无菌双头拭子对所有办公室共有的5个表面进行采样，一个用于培养，一个用于DNA提取。使用条形码“通用”细菌引物对54个表面（每个城市18个）的16 S rRNA基因序列进行PCR扩增，并合并进行焦磷酸测序。三因素方差分析（ANOVA）发现，男性或女性居住的办公室之间，各种表面类型之间以及城市之间的活细菌丰度存在显着差异。多重焦磷酸测序鉴定了来自20个不同细菌部门的500多个细菌属。这些属中最丰富的往往是人类皮肤，鼻腔，口腔或肠道的常见居民。其他常见的属似乎有环境起源（例如，土壤）。在男性或女性居住的办公室之间或表面之间，细菌多样性没有显着差异，但图森样本的细菌群落多样性与纽约和旧金山弗朗西斯科的细菌群落多样性有明显区别。总的来说，我们对办公楼微生物多样性的全面分子分析显示了这些方法用于研究室内细菌污染模式和起源的潜力。“人类在微生物的海洋中移动，除非在潜在的疾病和腐烂的背景下，否则很少被察觉。- Feazel等人（2009年）。
People in developed countries spend approximately 90% of their lives indoors, yet we know little about the source and diversity of microbes in built environments. In this study, we combined culture-based cell counting and multiplexed pyrosequencing of environmental ribosomal RNA (rRNA) gene sequences to investigate office space bacterial diversity in three metropolitan areas. Five surfaces common to all offices were sampled using sterile double-tipped swabs, one tip for culturing and one for DNA extraction, in 30 different offices per city (90 offices, 450 total samples). 16S rRNA gene sequences were PCR amplified using bar-coded “universal” bacterial primers from 54 of the surfaces (18 per city) and pooled for pyrosequencing. A three-factorial Analysis of Variance (ANOVA) found significant differences in viable bacterial abundance between offices inhabited by men or women, among the various surface types, and among cities. Multiplex pyrosequencing identified more than 500 bacterial genera from 20 different bacterial divisions. The most abundant of these genera tended to be common inhabitants of human skin, nasal, oral or intestinal cavities. Other commonly occurring genera appeared to have environmental origins (e.g., soils). There were no significant differences in the bacterial diversity between offices inhabited by men or women or among surfaces, but the bacterial community diversity of the Tucson samples was clearly distinguishable from that of New York and San Francisco, which were indistinguishable. Overall, our comprehensive molecular analysis of office building microbial diversity shows the potential of these methods for studying patterns and origins of indoor bacterial contamination. “[H]umans move through a sea of microbial life that is seldom perceived except in the context of potential disease and decay.” – Feazel et al. (2009).
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影响因子：
4.2
作者：
Benson, Courtney A.;Bizzoco, Richard W.;Kelley, Scott T.
通讯作者：
Kelley, Scott T.
DOI：
10.1073/pnas.0608255104
发表时间：
2007-01-02
影响因子：
11.1
作者：
Brodie, Eoin L.;DeSantis, Todd Z.;Andersen, Gary L.
通讯作者：
Andersen, Gary L.
影响因子：
2.5
作者：
Macpherson, Andrew J.;Slack, Emma
通讯作者：
Slack, Emma
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
14.9
作者：
Liu Z;Lozupone C;Hamady M;Bushman FD;Knight R
通讯作者：
Knight R