Genomic analysis of the carboxylesterase family in the salmon louse (Lepeophtheirus salmonis).
Genomic analysis of the carboxylesterase family in the salmon louse (Lepeophtheirus salmonis).
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DOI：
10.1016/j.cbpc.2021.109095
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Sturm A
中科院分区：
文献类型：
--
作者：
Tschesche C;Bekaert M;Humble JL;Bron JE;Sturm A
拟除虫菊酯溴氰菊酯和大环内酯甲氨基阿维菌素苯甲酸盐（EMB）用于治疗养殖鲑鱼受寄生鲑鱼虱（鲑虱）侵染。虽然这两种化合物对大西洋寄生虫种群的效力由于抗性的演变而降低，但L.鲑鱼目前还没有完全了解。羧酸酯酶（CaE）家族是一类功能多样的家族，其成员参与陆生节肢动物的农药抗性表型。本研究的目的是对L.沙门氏菌，并评估其在耐药性中的作用。L.通过在寄生虫的转录组和基因组中进行同源性搜索来鉴定鲑鱼CaE同源物。利用定量逆转录PCR技术研究了在敏感和多重耐药的L.鲑鱼上述策略导致21个CaEs基因/假基因的鉴定。系统发育分析分配13个钙调素参与神经发育信号和细胞粘附的分支，而三个序列被预测为编码分泌酶。10个CAE被确定为具有潜在的催化能力。乙酰胆碱酯酶（ace 1b）的转录表达显着增加，在多重耐药虱相比，药物敏感的L。鲑，转录丰度进一步增加，在preadult-II女性EMB曝光后。总之，本研究的结果表明，L。鲑鱼具有较少的CaE基因家族成员比大多数节肢动物的特点。L.鲑鱼与ACE 1B的过度表达有关。在L.鲑鱼ace 1b在敏感和耐药沙门氏菌中的差异表达在亚致死EMB浓度下调节ace 1b的转录表达没有证据表明CaEs在L.鲑鱼到DTM和EMB
The pyrethroid deltamethrin and the macrocyclic lactone emamectin benzoate (EMB) are used to treat infestations of farmed salmon by parasitic salmon lice, Lepeophtheirus salmonis. While the efficacy of both compounds against Atlantic populations of the parasite has decreased as a result of the evolution of resistance, the molecular mechanisms of drug resistance in L. salmonis are currently not fully understood. The functionally diverse carboxylesterases (CaE) family includes members involved in pesticide resistance phenotypes of terrestrial arthropods. The present study had the objective to characterize the CaE family in L. salmonis and assess its role in drug resistance. L. salmonis CaE homologues were identified by homology searches in the parasite's transcriptome and genome. The transcript expression of CaEs predicted to be catalytically competent was studied using quantitative reverse-transcription PCR in drug susceptible and multi-resistant L. salmonis. The above strategy led to the identification of 21 CaEs genes/pseudogenes. Phylogenetic analyses assigned 13 CaEs to clades involved in neurodevelopmental signaling and cell adhesion, while three sequences were predicted to encode secreted enzymes. Ten CaEs were identified as being potentially catalytically competent. Transcript expression of acetylcholinesterase (ace1b) was significantly increased in multi-resistant lice compared to drug-susceptible L. salmonis, with transcript abundance further increased in preadult-II females following EMB exposure. In summary, results from the present study demonstrate that L. salmonis possesses fewer CaE gene family members than most arthropods characterized so far. Drug resistance in L. salmonis was associated with overexpression of ace1b. 21 genes/pseudogenes coding for carboxylesterases (CaEs) identified in L. salmonis Differential expression of ace1b between drug susceptible and resistant L. salmonis Regulation of transcript expression of ace1b following sublethal EMB concentrations No evidence for key role of CaEs in resistance of L. salmonis to DTM and EMB
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影响因子：
3.7
作者：
Biswas S;Reinhard J;Oakeshott J;Russell R;Srinivasan MV;Claudianos C
通讯作者：
Claudianos C
果蝇的β-酯酶簇中的基因的出生和死亡。
影响因子：
3.9
作者：
Robin, Charles;Bardsley, Lisa M. J.;Coppin, Chris;Oakeshott, John G.
通讯作者：
Oakeshott, John G.
影响因子：
2.5
作者：
Costello, Mark J.
通讯作者：
Costello, Mark J.
影响因子：
4.5
作者：
Carmona-Antonanzas, Greta;Humble, Joseph L.;Carmichael, Stephen N.;Heumann, Jan;Christie, Hayden R. L.;Green, Darren M.;Bassett, David I.;Bron, James E.;Sturm, Armin
通讯作者：
Sturm, Armin
影响因子：
5.4
作者：
Chertemps T;François A;Durand N;Rosell G;Dekker T;Lucas P;Maïbèche-Coisne M
通讯作者：
Maïbèche-Coisne M