A carboxylesterase, Esterase-6, modulates sensory physiological and behavioral response dynamics to pheromone in Drosophila.
A carboxylesterase, Esterase-6, modulates sensory physiological and behavioral response dynamics to pheromone in Drosophila.
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DOI：
10.1186/1741-7007-10-56
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发表时间：
2012-06-21
期刊：
影响因子：
5.4
通讯作者：
Maïbèche-Coisne M
中科院分区：
文献类型：
--
作者：
Chertemps T;François A;Durand N;Rosell G;Dekker T;Lucas P;Maïbèche-Coisne M
昆虫对信息素羽流的空间和时间动态做出反应，这意味着不仅对“气味开启”有强烈反应，而且对“气味关闭”也有强烈反应。这需要针对快速信号终止的机制。多种机制可能有助于信号终止，其中包括气味降解酶。尽管缺乏直接的体内实验证据，但这些酶可能通过感觉受体附近的气味物质的快速失活而在信号动力学中发挥作用。在这里，我们验证了细胞外羧酸酯酶酯酶 6 (Est-6) 在果蝇对其信息素顺式乙酸乙酸酯 (cVA) 的感觉生理和行为动力学反应中的作用。 Est-6 此前曾被认为与雌性生殖系统的交配后效应有关。由于 Est-6 还可以在体外水解 cVA，并且在主要嗅觉器官（触角）中表达，因此我们在这里测试了它作为假定的气味降解酶在嗅觉中的作用。我们首先确认 Est-6 在嗅觉感觉器（包括 cVA 敏感感觉器）中高表达，并且我们表明该表达可能与非神经元细胞相关。我们的电生理学方法表明，嗅觉受体神经元 (ORN) 反应的动力学受到 Est-6 的强烈影响，如在 Est-6° 无效突变体（缺乏 Est-6 基因）中，cVA 敏感的 ORN 显示出对 cVA 的放电率增加和活性延长。对两种 cVA 诱导行为的分析表明，Est-6° 突变雄性对 cVA 的行为反应阈值较低。特别是，突变的雄性表现出雄性求爱的强烈减少，这与求偶开始的延迟有关。我们的研究提供了证据，证明 Est-6 在雄性果蝇性信息素反应的生理和行为动力学中发挥作用，并支持 Est-6 作为雄性触角中的气味降解酶 (ODE) 的作用。我们的结果还扩展了 Est-6 在果蝇生物学中的作用，从繁殖到嗅觉，并强调了 ODE 在昆虫嗅觉中的作用。
Insects respond to the spatial and temporal dynamics of a pheromone plume, which implies not only a strong response to 'odor on', but also to 'odor off'. This requires mechanisms geared toward a fast signal termination. Several mechanisms may contribute to signal termination, among which odorant-degrading enzymes. These enzymes putatively play a role in signal dynamics by a rapid inactivation of odorants in the vicinity of the sensory receptors, although direct in vivo experimental evidences are lacking. Here we verified the role of an extracellular carboxylesterase, esterase-6 (Est-6), in the sensory physiological and behavioral dynamics of Drosophila melanogaster response to its pheromone, cis-vaccenyl acetate (cVA). Est-6 was previously linked to post-mating effects in the reproductive system of females. As Est-6 is also known to hydrolyze cVA in vitro and is expressed in the main olfactory organ, the antenna, we tested here its role in olfaction as a putative odorant-degrading enzyme. We first confirm that Est-6 is highly expressed in olfactory sensilla, including cVA-sensitive sensilla, and we show that expression is likely associated with non-neuronal cells. Our electrophysiological approaches show that the dynamics of olfactory receptor neuron (ORN) responses is strongly influenced by Est-6, as in Est-6° null mutants (lacking the Est-6 gene) cVA-sensitive ORN showed increased firing rate and prolonged activity in response to cVA. Est-6° mutant males had a lower threshold of behavioral response to cVA, as revealed by the analysis of two cVA-induced behaviors. In particular, mutant males exhibited a strong decrease of male-male courtship, in association with a delay in courtship initiation. Our study presents evidence that Est-6 plays a role in the physiological and behavioral dynamics of sex pheromone response in Drosophila males and supports a role of Est-6 as an odorant-degrading enzyme (ODE) in male antennae. Our results also expand the role of Est-6 in Drosophila biology, from reproduction to olfaction, and highlight the role of ODEs in insect olfaction.
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影响因子：
3.7
作者：
Durand N;Carot-Sans G;Bozzolan F;Rosell G;Siaussat D;Debernard S;Chertemps T;Maïbèche-Coisne M
通讯作者：
Maïbèche-Coisne M
DOI：
10.1073/pnas.0505340102
发表时间：
2005-09-27
影响因子：
11.1
作者：
Ishida, Y;Leal, WS
通讯作者：
Leal, WS
DOI：
10.1073/pnas.0802610105
发表时间：
2008-07-01
影响因子：
11.1
作者：
Ishida, Yuko;Leal, Walter S.
通讯作者：
Leal, Walter S.
影响因子：
64.8
作者：
Kurtovic, Amina;Widmer, Alexandre;Dickson, Barry J.
通讯作者：
Dickson, Barry J.
影响因子：
64.8
作者：
Benton, Richard;Vannice, Kirsten S.;Vosshall, Leslie B.
通讯作者：
Vosshall, Leslie B.