Mechanical changes in human dental pulp stem cells during early odontogenic differentiation.
Mechanical changes in human dental pulp stem cells during early odontogenic differentiation.
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DOI：
10.1016/j.joen.2014.07.030
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发表时间：
2015-01
影响因子：
4.2
通讯作者：
Alapati SB
中科院分区：
文献类型：
--
作者：
Jones TD;Naimipour H;Sun S;Cho M;Alapati SB
细胞在生物活性支架中的粘附和迁移需要肌动蛋白细胞骨架的重塑和粘着斑的形成。此外，人牙髓干细胞（hDPSC）在牙源性分化过程中其机械性能发生了一些变化。牙形成所必需的因素对肌动蛋白应力纤维弹性和粘着斑形成的影响尚不清楚。使用原子力显微镜对在牙源性培养基中培养的活的hDPSC的细胞骨架变化进行成像。记录细胞骨架的杨氏模量（kPa）作为培养10天的培养基的函数。使用免疫荧光评估粘着斑形成。将培养的hDPSC与单克隆黏着斑蛋白抗体孵育，并使用0.5 μM鬼笔环肽使丝状肌动蛋白可视化。细胞骨架弹性显着增加牙源性介质。hDPSC中局灶性粘连的数量和物理尺寸也增加。黏着斑蛋白表达上调明显。局灶性粘连形成的增加与肌动蛋白重塑应力纤维一致。我们的研究结果表明，hDPSCs牢固地附着在玻璃基板上，以响应牙源性介质。使用仿生支架的牙髓牙本质组织的成功再生可能需要受生化诱导因子影响的细胞-细胞外基质（ECM）相互作用。
Cell adhesion and migration in bioactive scaffolds require actin cytoskeleton remodeling and focal adhesion formation. Additionally, human dental pulp stem cells (hDPSCs) undergo several changes in their mechanical properties during odontogenic differentiation. The effect of factors essential for odontogenesis on the actin stress fiber elasticity and focal adhesion formation is not known. Live hDPSCs cultured in odontogenic media were imaged for cytoskeleton changes using an atomic force microscope. The Young’s modulus (kPa) of the cytoskeleton was recorded as a function of culture medium for 10 days. Focal adhesion formation was assessed using immunofluorescence. Cultured hDPSCs were incubated with a monoclonal vinculin antibody and filamentous actins were visualized using 0.5 µM phalloidin. Cytoskeletal elasticity significantly increased in response to odontogenic media. Both the number and physical size of focal adhesions in hDPSCs also increased. Up-regulation of vinculin expression was evident. The increase in the formation of focal adhesions was consistent with actin remodeling to stress fibers. Our findings suggest that hDPSCs firmly attach to the glass substrate in response to odontogenic media. Successful regeneration of pulp-dentin tissue using biomimetic scaffolds will likely require cell-extracellular matrix (ECM) interactions influenced by biochemical induction factors.
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影响因子：
4.1
作者：
Shi, S;Robey, PG;Gronthos, S
通讯作者：
Gronthos, S
影响因子：
4
作者：
Zaidel-Bar, R;Ballestrem, C;Geiger, B
通讯作者：
Geiger, B
DOI：
10.1016/0922-3371(90)90048-2
发表时间：
1990-12-01
期刊：
CELL DIFFERENTIATION AND DEVELOPMENT
影响因子：
--
作者：
BURRIDGE, K;NUCKOLLS, G;TURNER, C
通讯作者：
TURNER, C
DOI：
10.1006/scel.1994.1021
发表时间：
1994-01-01
期刊：
Seminars in Cell Biology
影响因子：
--
作者：
Matsudaira, Paul
通讯作者：
Matsudaira, Paul
影响因子：
--
作者：
Mao JJ;Kim SG;Zhou J;Ye L;Cho S;Suzuki T;Fu SY;Yang R;Zhou X
通讯作者：
Zhou X