The rumen microbiome as a reservoir of antimicrobial resistance and pathogenicity genes is directly affected by diet in beef cattle.
The rumen microbiome as a reservoir of antimicrobial resistance and pathogenicity genes is directly affected by diet in beef cattle.
复制标题
DOI：
10.1186/s40168-017-0378-z
复制
发表时间：
2017-12-11
期刊：
影响因子：
15.5
通讯作者：
Roehe R
中科院分区：
文献类型：
--
作者：
Auffret MD;Dewhurst RJ;Duthie CA;Rooke JA;John Wallace R;Freeman TC;Stewart R;Watson M;Roehe R
抗菌素耐药性的出现和蔓延是当前对人类和动物健康最紧迫的威胁。更好地了解动物肠道中大量的抗菌素耐药性基因以及与微生物定植和致病性相关的基因，将在减少动物生产对这一问题的影响方面发挥重要作用。在此，我们在取自 50 头不含抗生素的肉牛的瘤胃食糜样本中评估了饮食对瘤胃抗性组和致病性基因丰度的影响，其中包括接受两种含有不同比例浓缩物的饮食的四个牛品种。使用 KEGG 鉴定，从 4966 个宏基因组基因中鉴定出 204 个与抗菌素耐药性 (AMR)、定植、通讯或致病功能相关的基因。在集中喂养的动物中，这些基因的多样性和丰度都较高。氯霉素和小菌素抗性基因在草料喂养的动物样本中占主导地位（P<0.001），而氨基糖苷类和链霉素抗性在精料喂养的动物中富集。以浓缩物为主的饮食还增加了变形菌的相对丰度，其中包括许多动物和人畜共患病原体。变形菌门与（厚壁菌门 + 拟杆菌门）的高比例被证实是瘤胃生态失调的良好指标，其中 8 个病例均来自精料喂养的动物。最后，网络分析表明，抗性/致病性基因可作为瘤胃微生物组健康风险评估的生物标志物。饮食对瘤胃微生物群落中 AMR 基因的补充具有重要影响，对人类和动物健康具有潜在影响。本文的在线版本 (10.1186/s40168-017-0378-z) 包含补充材料，可供授权用户使用。
The emergence and spread of antimicrobial resistance is the most urgent current threat to human and animal health. An improved understanding of the abundance of antimicrobial resistance genes and genes associated with microbial colonisation and pathogenicity in the animal gut will have a major role in reducing the contribution of animal production to this problem. Here, the influence of diet on the ruminal resistome and abundance of pathogenicity genes was assessed in ruminal digesta samples taken from 50 antibiotic-free beef cattle, comprising four cattle breeds receiving two diets containing different proportions of concentrate. Two hundred and four genes associated with antimicrobial resistance (AMR), colonisation, communication or pathogenicity functions were identified from 4966 metagenomic genes using KEGG identification. Both the diversity and abundance of these genes were higher in concentrate-fed animals. Chloramphenicol and microcin resistance genes were dominant in samples from forage-fed animals (P < 0.001), while aminoglycoside and streptomycin resistances were enriched in concentrate-fed animals. The concentrate-based diet also increased the relative abundance of Proteobacteria, which includes many animal and zoonotic pathogens. A high ratio of Proteobacteria to (Firmicutes + Bacteroidetes) was confirmed as a good indicator for rumen dysbiosis, with eight cases all from concentrate-fed animals. Finally, network analysis demonstrated that the resistance/pathogenicity genes are potentially useful as biomarkers for health risk assessment of the ruminal microbiome. Diet has important effects on the complement of AMR genes in the rumen microbial community, with potential implications for human and animal health. The online version of this article (10.1186/s40168-017-0378-z) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
8
作者：
通讯作者：
--
影响因子：
3.7
作者：
Green ER;Mecsas J
通讯作者：
Mecsas J
影响因子：
3.5
作者：
Friggens, NC;Oldham, JD;Horgan, G
通讯作者：
Horgan, G
影响因子：
3.6
作者：
Duthie, C. -A.;Troy, S. M.;Rooke, J. A.
通讯作者：
Rooke, J. A.
影响因子：
2.8
作者：
Hejnova, J;Dobrindt, U;Buchrieser, C
通讯作者：
Buchrieser, C