Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis.
Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis.
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DOI：
10.1038/srep35461
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发表时间：
2016-10-17
影响因子：
4.6
通讯作者：
Irigoien X
中科院分区：
文献类型：
--
作者：
Casas L;Saborido-Rey F;Ryu T;Michell C;Ravasi T;Irigoien X
顺序两性是硬骨鱼独特的繁殖策略，主要表现在生活在珊瑚礁环境中的鱼类物种中。两性动物的生殖生物学长期以来一直很耐人寻味；然而，人们对其性别变化背后的分子途径知之甚少。在这里，我们提供了首次对两性硬骨鱼S在其自然环境中经历性别变化的全新转录组分析。我们的研究检查了多个组的相对基因表达--而不仅仅是两个相反的条件--并使我们能够探索整个过程中的差异表达模式。我们的分析突出了大脑与性别变化相关的快速而复杂的基因组反应，这些反应随后被传递到性腺，识别出大量参与这一过程的候选基因，其中一些是众所周知的，另一些是新的。这项研究提供了强有力的证据，证明了性类固醇合成机制在小丑鱼性别转变过程中的重要性，芳香酶基因在大脑和性腺中都发挥着核心作用。这项工作构成了社会性别变化物种的第一个全基因组研究，并为控制两性原始人的社会性别变化和性腺重组的遗传机制提供了见解。
Sequential hermaphroditism is a unique reproductive strategy among teleosts that is displayed mainly in fish species living in the coral reef environment. The reproductive biology of hermaphrodites has long been intriguing; however, very little is known about the molecular pathways underlying their sex change. Here, we provide the first de novo transcriptome analyses of a hermaphrodite teleost´s undergoing sex change in its natural environment. Our study has examined relative gene expression across multiple groups—rather than just two contrasting conditions— and has allowed us to explore the differential expression patterns throughout the whole process. Our analysis has highlighted the rapid and complex genomic response of the brain associated with sex change, which is subsequently transmitted to the gonads, identifying a large number of candidate genes, some well-known and some novel, involved in the process. The present study provides strong evidence of the importance of the sex steroidogenic machinery during sex change in clownfish, with the aromatase gene playing a central role, both in the brain and the gonad. This work constitutes the first genome-wide study in a social sex-changing species and provides insights into the genetic mechanism governing social sex change and gonadal restructuring in protandrous hermaphrodites.
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DOI：
10.1016/j.beem.2009.12.002
发表时间：
2010-04-01
影响因子：
7.4
作者：
Biason-Lauber, Anna
通讯作者：
Biason-Lauber, Anna
影响因子：
3.6
作者：
Chaves-Pozo, E.;Liarte, S.;Garcia-Ayala, A.
通讯作者：
Garcia-Ayala, A.
影响因子：
4.9
作者：
Champy, M-F;Le Voci, L.;Blom, D.
通讯作者：
Blom, D.
影响因子：
1.7
作者：
Alonso-Fernandez, Alexandre;Alos, Josep;Saborido-Rey, Fran
通讯作者：
Saborido-Rey, Fran
影响因子：
64.8
作者：
Buston, P
通讯作者：
Buston, P