Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members.
Comparing gut resistome composition among patients with acute Campylobacter infections and healthy family members.
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DOI：
10.1038/s41598-021-01927-7
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发表时间：
2021-11-16
影响因子：
4.6
通讯作者：
Manning SD
中科院分区：
文献类型：
--
作者：
Hansen ZA;Cha W;Nohomovich B;Newton DW;Lephart P;Salimnia H;Khalife W;Shade A;Rudrik JT;Manning SD
弯曲杆菌通常引起食源性感染，抗生素耐药性是一个迫在眉睫的问题。然而，目前尚不清楚人类肠道“抵抗体”在感染期间是否受到弯曲杆菌的影响。鸟枪宏基因组学对来自26例弯曲杆菌感染病例和44名健康家庭成员（对照）的粪便的应用鉴定了406个独特的抗生素耐药基因（ARG），代表153个基因/操纵子，40种机制和18个类别。病例组的ARG丰富度（p < 0.0001）和Shannon多样性（p < 0.0001）高于具有不同组成的对照组（p = 0.000999; PERMANOVA）。病例由多药耐药基因定义，以变形菌门（40.8%）为主，特别是代表埃希氏菌（20.9%）。四环素耐药基因在对照组中最丰富，以拟杆菌门（45.3%）和厚壁菌门（44.4%）为主。病例的分层聚类确定了具有不同耐药的三个聚类。病例群1和3与包含更多城市和住院患者的对照组不同。相对于同一家庭的家庭成员，匹配病例中的ARG组成大多是不同的，尽管一些家庭对照具有相似的特征，这可以解释为自暴露于病例以来的时间较短。总之，这些数据表明弯曲杆菌感染与耐药基因组组成的改变和ARG多样性的增加有关，引起了人们对感染在耐药决定因素传播中的作用的担忧。
Campylobacter commonly causes foodborne infections and antibiotic resistance is an imminent concern. It is not clear, however, if the human gut ‘resistome’ is affected by Campylobacter during infection. Application of shotgun metagenomics on stools from 26 cases with Campylobacter infections and 44 healthy family members (controls) identified 406 unique antibiotic resistance genes (ARGs) representing 153 genes/operons, 40 mechanisms, and 18 classes. Cases had greater ARG richness (p < 0.0001) and Shannon diversity (p < 0.0001) than controls with distinct compositions (p = 0.000999; PERMANOVA). Cases were defined by multidrug resistance genes and were dominated by Proteobacteria (40.8%), specifically those representing Escherichia (20.9%). Tetracycline resistance genes were most abundant in controls, which were dominated by Bacteroidetes (45.3%) and Firmicutes (44.4%). Hierarchical clustering of cases identified three clusters with distinct resistomes. Case clusters 1 and 3 differed from controls containing more urban and hospitalized patients. Relative to family members of the same household, ARG composition among matched cases was mostly distinct, though some familial controls had similar profiles that could be explained by a shorter time since exposure to the case. Together, these data indicate that Campylobacter infection is associated with an altered resistome composition and increased ARG diversity, raising concerns about the role of infection in the spread of resistance determinants.
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DOI：
10.1093/cid/ciw542
发表时间：
2016-11-01
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Amour C;Gratz J;Mduma E;Svensen E;Rogawski ET;McGrath M;Seidman JC;McCormick BJ;Shrestha S;Samie A;Mahfuz M;Qureshi S;Hotwani A;Babji S;Trigoso DR;Lima AA;Bodhidatta L;Bessong P;Ahmed T;Shakoor S;Kang G;Kosek M;Guerrant RL;Lang D;Gottlieb M;Houpt ER;Platts-Mills JA;Etiology, Risk Factors, and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development Project (MAL-ED) Network Investigators
通讯作者：
Etiology, Risk Factors, and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development Project (MAL-ED) Network Investigators
影响因子：
5.2
作者：
John DA;Williams LK;Kanamarlapudi V;Humphrey TJ;Wilkinson TS
通讯作者：
Wilkinson TS
对大量人类肠道微生物群中抗生素抗性基因进行宏基因组分析
影响因子：
16.6
作者：
Hu, Yongfei;Yang, Xi;Zhu, Baoli
通讯作者：
Zhu, Baoli
DOI：
10.1126/science.1220761
发表时间：
2012-08-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Forsberg KJ;Reyes A;Wang B;Selleck EM;Sommer MO;Dantas G
通讯作者：
Dantas G
影响因子：
3
作者：
Lozupone C;Hamady M;Knight R
通讯作者：
Knight R