Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes.
Molecular characterisation of Staphylococcus aureus in school-age children in Guangzhou: associations among agr types, virulence genes, sequence types, and antibiotic resistant phenotypes.
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DOI：
10.1186/s12866-023-03126-y
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发表时间：
2023-11-29
期刊：
影响因子：
4.2
通讯作者：
Liang B
中科院分区：
文献类型：
--
作者：
Cai H;Li X;Zhang C;Zhong H;Xie Y;Huang L;Zhang B;Long Y;Zhou Z;Liang B
金黄色葡萄球菌是最普遍的机会致病菌之一，主要定植于鼻腔，是严重感染的危险因素。毒力因子和辅助基因调节因子（agr）是决定葡萄球菌感染严重性和多样性的关键。在这项研究中，我们的目标是，aureus agr基因型和毒力基因，并与遗传背景和耐药表型相关。704株（98.5%）分离到agr型，仅11株为阴性。我们的大多数分离物被归类为agr I型，其次是III型，II型和IV型。肠毒素c基因（sec）在48.6%的分离株中检出，在检出的5种肠毒素基因中检出率最高。lukS/F-PV和tsst基因的阳性率分别为4%和2.2%，而sed和SasX均未检测到。ST 45、ST 59、ST 338、ST 188、ST 6、ST 7、ST 22、ST 25、ST 398和ST 944属于agr Ⅰ群，ST 5和ST 15属于agr Ⅱ群。ST 30和ST 1为Ⅲ级组，ST 121为Ⅳ级组。tsst基因仅在属于ST 7和ST 30分离株的agr I和III型中发现，而lukS/F-PV主要由agr I型分离株携带，主要在CC 59和CC 22克隆中。在耐甲氧西林的S.金黄色葡萄球菌（MRSA）中89.7%属于agr Ⅰ群，97.8%的利福平耐药或中间耐药菌株属于agr Ⅰ群。MRSA分离株比甲氧西林敏感的S.金黄色葡萄球菌分离株分析了715 S的agr型和8个主要毒力基因的分布。金黄色葡萄球菌分离株，我们的研究结果揭示了明确的关联agr类型和ST，以及毒力基因，耐药表型。
Staphylococcus aureus, one of the most prevalent opportunistic pathogens, mainly colonizes the nasal cavity and is a risk factor for severe infections. Virulence factors and accessory gene regulator (agr) are key to the severity and diversity of staphylococcal infection. In this study, we aimed to characterise S. aureus agr-types and virulence genes and correlated them with genetic background and antibiotic-resistant phenotypes. Agr types were identified in 704 isolates (98.5%), with only 11 isolates were negative for agr type. Most of our isolates were classified as agr type I, followed by types III, II and IV. The enterotoxin c gene (sec) was detected in 48.6% of isolates, showing the highest prevalence among the five enterotoxin genes detected. The positivity rates for the lukS/F-PV and tsst genes were 4% and 2.2%, respectively, while neither sed nor SasX were detected. ST45, ST59, ST338, ST188, ST6, ST7, ST22, ST25, ST398, and ST944 belonged to agr I group, while ST5 and ST15 belonged to agr II group. ST30 and ST1 were classified into agr III group, and ST121 was assigned into agr IV group. The tsst gene was found exclusively within agr I and III types belonging to ST7 and ST30 isolates, while the lukS/F-PV was predominantly carried by agr I type isolates primarily within CC59 and CC22 clones. Among the methicillin-resistant S. aureus (MRSA) isolates, 89.7% belonged to agr I group, and 97.8% of rifampicin-resistant or intermediate isolates were assigned to agr I group. MRSA isolates harboured more tested virulence genes compared to methicillin-susceptible S. aureus isolates. We characterized the distributions of agr types and eight major virulence genes of 715 S. aureus isolates, and our findings revealed clear associations between agr types and STs, as well as virulence genes, and drug resistant phenotypes.
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