Detection of Bourbon Virus-Specific Serum Neutralizing Antibodies in Human Serum in Missouri, USA.
Detection of Bourbon Virus-Specific Serum Neutralizing Antibodies in Human Serum in Missouri, USA.
复制标题
作者：
波旁病毒（BRBV）于2014年首次在一个致命的人类病例中被发现。从那时起，在美国密苏里州和堪萨斯州的蜱虫中发现了这种病毒。尽管这些州蜱中BRBV的流行率很高，但报告的人间病例很少，社区中BRBV的真正感染负担尚不清楚。在这里，我们开发了两种病毒中和试验，一种是水疱性口炎病毒(VSV)-BRBV伪型快速检测，另一种是BRBV减灶中和试验，以评估2020年在密苏里州圣路易斯收集的人类血清中BRBV中和抗体的血清阳性率。在440份检测的人类血清样本中，3份（0.7%）能够有效中和VSV-BRBV和野生型BRBV。这些发现表明，人类感染BRBV比以前认识到的更为常见。自2014年发现波旁病毒（BRBV）以来，共发现了5例人间病例，包括2例死亡病例。BRBV被认为是由在美国东部、东南部和中西部流行的孤星蜱传播的。在密苏里州和堪萨斯州的蜱虫中发现了BRBV，血清学证据表明它也存在于北卡罗来纳州。然而，人类BRBV的真正感染负担尚不清楚。在本研究中，我们开发了两种病毒中和试验，以评估2020年在密苏里州圣路易斯收集的人类血清中BRBV特异性抗体的血清阳性率。我们发现一小部分个体的BRBV中和抗体呈血清阳性。我们的数据表明，人类BRBV感染比以前认为的更常见。
Bourbon virus (BRBV) was first discovered in 2014 in a fatal human case. Since then it has been detected in the tick Amblyomma americanum in the states of Missouri and Kansas in the United States. Despite the high prevalence of BRBV in ticks in these states, very few human cases have been reported, and the true infection burden of BRBV in the community is unknown. Here, we developed two virus neutralization assays, a vesicular stomatitis virus (VSV)-BRBV pseudotyped rapid assay and a BRBV focus reduction neutralization assay, to assess the seroprevalence of BRBV neutralizing antibodies in human sera collected in 2020 in St. Louis, MO. Of 440 human serum samples tested, three (0.7%) were able to potently neutralize both VSV-BRBV and wild-type BRBV. These findings suggest that human infections with BRBV are more common than previously recognized. IMPORTANCE Since the discovery of the Bourbon virus (BRBV) in 2014, a total of five human cases have been identified, including two fatal cases. BRBV is thought to be transmitted by the lone star tick, which is prevalent in the eastern, southeastern, and midwestern United States. BRBV has been detected in ticks in Missouri and Kansas, and serological evidence suggests that it is also present in North Carolina. However, the true infection burden of BRBV in humans is not known. In the present study, we developed two virus neutralization assays to assess the seroprevalence of BRBV-specific antibodies in human sera collected in 2020 in St. Louis, MO. We found that a small subset of individuals are seropositive for neutralizing antibodies against BRBV. Our data suggest that BRBV infection in humans is more common than previously thought.
登录
查看更多内容
影响因子：
11.8
作者：
Savage HM;Burkhalter KL;Godsey MS Jr;Panella NA;Ashley DC;Nicholson WL;Lambert AJ
通讯作者：
Lambert AJ
影响因子：
30.3
作者：
Case, James Brett;Rothlauf, Paul W.;Whelan, Sean P. J.
通讯作者：
Whelan, Sean P. J.
美国密苏里州圣路易斯儿童和成人中SARS-COV-2抗体的血清阳性。
影响因子：
4.8
作者：
Smith BK;Janowski AB;Danis JE;Harvey IB;Zhao H;Dai YN;Farnsworth CW;Gronowski AM;Roper S;Fremont DH;Wang D
通讯作者：
Wang D
影响因子：
8.8
作者：
Lambert, Amy J.;Velez, Jason O.;Kosoya, Olga I.
通讯作者：
Kosoya, Olga I.
影响因子：
11.8
作者：
Lindsey, Nicole P.;Menitove, Jay E.;Staples, J. Erin
通讯作者：
Staples, J. Erin