Ex Vivo Plasmodium malariae Culture Method for Antimalarial Drugs Screen in the Field.
Ex Vivo Plasmodium malariae Culture Method for Antimalarial Drugs Screen in the Field.
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DOI：
10.1021/acsinfecdis.1c00262
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发表时间：
2021-11-12
影响因子：
5.3
通讯作者：
Djimde, Abdoulaye A.
中科院分区：
文献类型：
--
作者：
Dembele, Laurent;Diallo, Nouhoum;Sogore, Fanta;Diarra, Bintou;Ballo, Fatoumata, I;Daou, Amadou;Diakite, Ousmaila;Bare, Yacouba;Sangare, Cheick Papa Oumar;Haidara, Aboubecrin Sedhigh;Diakite, Seidina A. S.;Niangaly, Amadou;Diakite, Mahamadou;Campo, Brice;Awandare, Gordon A.;Aniweh, Yaw;Djimde, Abdoulaye A.
恶性疟原虫的体外和体外培养促进了对疟疾寄生虫的积极研究，以寻求基础知识和发现有效的药物治疗方法。这样的药物发现计划目前对疟疾原虫来说是困难的，因为缺乏支持抗疟疾评估的无性血液阶段的体外和体外培养系统。尽管有对恶性疟原虫有效的青蒿素联合疗法，但在疟疾流行国家中，疟疾正越来越多地被发现。疟原虫是慢性感染的罪魁祸首，并与贫血和发病率的高负担有关。在这里，我们优化和适应了体外培养疟疾原虫的条件，并将其用于筛选抗疟疾药物。随后，这使我们能够测试化合物，如蒿甲醚、氯喹、鲁米芬和奎宁的体外抗疟疾活性。
In vitro and ex vivo cultivation of Plasmodium (P) falciparum has facilitated active research into the malaria parasite toward the quest for basic knowledge and the discovery of effective drug treatments. Such a drug discovery program is currently difficult for P. malariae simply because of the absence of in vitro and ex vivo cultivation system for its asexual blood stages supporting antimalarial evaluation. Despite availability of artemisinin combination therapies effective on P. falciparum, P. malariae is being increasingly detected in malaria endemic countries. P. malariae is responsible for chronic infections and is associated with a high burden of anemia and morbidity. Here, we optimized and adapted ex vivo conditions under which P. malariae can be cultured and used for screening antimalarial drugs. Subsequently, this enabled us to test compounds such as artemether, chloroquine, lumefantrine, and quinine for ex vivo antimalarial activity against P. malariae.
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影响因子：
12.8
作者：
Bei, Amy K.;DeSimone, Tiffany M.;Badiane, Aida S.;Ahouidi, Ambroise D.;Dieye, Tandakha;Ndiaye, Daouda;Sarr, Ousmane;Ndir, Omar;Mboup, Souleymane;Duraisingh, Manoj T.
通讯作者：
Duraisingh, Manoj T.
影响因子：
11.8
作者：
Rutledge, Gavin G.;Marr, Ian;Price, Ric N.
通讯作者：
Price, Ric N.
影响因子：
6.4
作者：
Grignard, Lynn;Shah, Sonal;Fornace, Kimberly M.
通讯作者：
Fornace, Kimberly M.
DOI：
10.1093/infdis/jiy519
发表时间：
2019-02-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Imwong M;Madmanee W;Suwannasin K;Kunasol C;Peto TJ;Tripura R;von Seidlein L;Nguon C;Davoeung C;Day NPJ;Dondorp AM;White NJ
通讯作者：
White NJ
影响因子：
0.8
作者：
Kugasia, Irfanali R.;Polara, Farhana K.;Assallum, Hussein
通讯作者：
Assallum, Hussein