Arabidopsis cryptochrome 1 functions in nitrogen regulation of flowering
Arabidopsis cryptochrome 1 functions in nitrogen regulation of flowering
复制标题
拟南芥隐花色素1在开花氮调节中的作用
DOI：
10.1073/pnas.1602004113
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发表时间：
2016-06
影响因子：
11.1
通讯作者：
He, Yikun
中科院分区：
文献类型：
--
作者：
Kong, Dong-Dong;Xiao, Wei;Lin, Hong-Hui;He, Yikun
Significance Overapplication of nitrogen (N) fertilizer causes delayed flowering and negatively impacts the function and composition of natural ecosystems and climate. In this study, we demonstrate that flowering time variations regulated by altered nitrogen levels are mediated by two key factors: ferredoxin-NADP+-oxidoreductase (FNR1) and the blue-light receptor cryptochrome 1 (CRY1). Nitrogen regulates FNR1 expression, thereby contributing to changes in NADPH/NADP+ and ATP/AMP ratios, which in turn activates adenosine monophosphate-activated protein kinase to modulate nuclear CRY1 abundance, which further acts in the N signal input pathway to affect central clock function and flowering time. A better understanding of N-regulated floral transition will offer biotechnological solutions to improve sustainable agriculture. The phenomenon of delayed flowering after the application of nitrogen (N) fertilizer has long been known in agriculture, but the detailed molecular basis for this phenomenon is largely unclear. Here we used a modified method of suppression-subtractive hybridization to identify two key factors involved in N-regulated flowering time control in Arabidopsis thaliana, namely ferredoxin-NADP+-oxidoreductase and the blue-light receptor cryptochrome 1 (CRY1). The expression of both genes is induced by low N levels, and their loss-of-function mutants are insensitive to altered N concentration. Low-N conditions increase both NADPH/NADP+ and ATP/AMP ratios, which in turn affect adenosine monophosphate-activated protein kinase (AMPK) activity. Moreover, our results show that the AMPK activity and nuclear localization are rhythmic and inversely correlated with nuclear CRY1 protein abundance. Low-N conditions increase but high-N conditions decrease the expression of several key components of the central oscillator (e.g., CCA1, LHY, and TOC1) and the flowering output genes (e.g., GI and CO). Taken together, our results suggest that N signaling functions as a modulator of nuclear CRY1 protein abundance, as well as the input signal for the central circadian clock to interfere with the normal flowering process.
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影响因子：
5.6
作者：
Crozet P;Margalha L;Confraria A;Rodrigues A;Martinho C;Adamo M;Elias CA;Baena-González E
通讯作者：
Baena-González E
影响因子：
11.6
作者：
Huang-Lung Tsai;Yi-Hang Li;Wen-Ping Hsieh;Meng-Chun Lin;J. Ahn;Shu-Hsing Wu
通讯作者：
Huang-Lung Tsai;Yi-Hang Li;Wen-Ping Hsieh;Meng-Chun Lin;J. Ahn;Shu-Hsing Wu
影响因子：
56.9
作者：
Valverde, F;Mouradov, A;Coupland, G
通讯作者：
Coupland, G
DOI：
10.1105/tpc.010126
发表时间：
2001-08
期刊：
The Plant Cell Online
影响因子：
--
作者：
Fang-Qing Guo;Rongchen Wang;Mingsheng Chen;N. Crawford
通讯作者：
Fang-Qing Guo;Rongchen Wang;Mingsheng Chen;N. Crawford
影响因子：
56.9
作者：
Liu, Hongtao;Yu, Xuhong;Lin, Chentao
通讯作者：
Lin, Chentao