A long non-coding apple RNA, MSTRG.85814.11, acts as a transcriptional enhancer of SAUR32 and contributes to the Fe deficiency response.
A long non-coding apple RNA, MSTRG.85814.11, acts as a transcriptional enhancer of SAUR32 and contributes to the Fe deficiency response.
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一种长的非编码苹果 RNA，MSTRG.85814.11，作为 SAUR32 的转录增强子，有助于铁缺乏反应。
DOI：
10.1111/tpj.14706
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发表时间：
2020-01
期刊：
影响因子：
7.2
通讯作者：
Wu Ting
中科院分区：
文献类型：
--
作者：
Sun Yaqiang;Hao Pengbo;Lv Xinmin;Tian Ji;Wang Yi;Zhang Xinzhong;Xu Xuefeng;Han Zhenhai;Wu Ting
铁（Fe）是植物必需的营养素，其缺乏通常限制植物生长。长非编码（lnc）-RNA参与营养胁迫的适应性反应，然而，目前尚不清楚它们是否在典型的铁缺乏反应的调节功能。苹果（Malus acutica）lncRNA，MSTRG. 85814的表达由Fe缺乏诱导，如通过苹果同源嫁接系统的高通量链特异性RNA-seq分析所鉴定。MSTRG. 85814具有复杂的结构，具有13种预测的RNA序列变体，其中4种在经历Fe缺乏的植物的根中上调。我们发现，一个MSTRG.85814剪接变异体（MSTRG.85814.11）正调制其顺式靶mRNA来自小生长素上调基因SAUR 32。这反过来又促进了SAUR 32的表达，并导致质膜质子ATP酶AHA 10的表达增加。使用pH成像技术，观察到在MSTRG. 85814. 11或SAUR 32过表达的苹果植物的根尖中发生质外体pH的显著降低。因此，MSTRG. 85814. 11显示出积极促进SAUR 32表达，其然后激活质子挤出以参与Fe缺乏反应。这些结果揭示了lncRNA促进环境缺铁胁迫适应的机制。
Iron (Fe) is an essential plant nutrient and its deficiency typically limits plant growth. Long non-coding (lnc)-RNAs are involved in adaptive responses to nutrient stresses; however, it is not known whether they function in the regulation of the canonical Fe deficiency response. The expression of apple (Malus domestica) lncRNA, MSTRG.85814 is induced by Fe deficiency, as identified by high-throughput strand-specific RNA-seq analysis of an apple homo-graft system. MSTRG.85814 has a complex structure, with 13 predicted RNA sequence variants, four of which are upregulated in the roots of plants experiencing Fe deficiency. We found that one MSTRG.85814 splice variant (MSTRG.85814.11) positively modulated its cis target mRNA derived from the small auxin up-regulated gene SAUR32. This in turn promoted the expression of SAUR32 and caused an increase in the expression of a plasma membrane proton ATPase, AHA10. Using a pH imaging technique, a significant decrease in the apoplastic pH was observed to occur in the root tips of MSTRG.85814.11 or SAUR32 overexpressing apple plants. Thus, MSTRG.85814.11 was shown to positively promote SAUR32 expression, which then activated proton extrusion to involve in the Fe deficiency response. These results reveal a mechanism by which lncRNA promotes environmental Fe deficiency stress adaption.
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影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
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影响因子：
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作者：
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
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影响因子：
14.9
作者：
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