A versatile system for detecting transposition in Arabidopsis.
A versatile system for detecting transposition in Arabidopsis.
复制标题
用于检测拟南芥转座的多功能系统。
DOI：
10.1111/j.1365-313x.1993.tb00178.x
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
David L. Smith
中科院分区：
文献类型：
--
作者：
N. Fedoroff;David L. Smith
玉米转座元件激活剂 (Ac) 已被证明在许多双子叶植物中具有活性，包括拟南芥，其基因组小和世代时间短，有利于其广泛采用作为植物生理学和发育分子遗传学方法的模式生物。利用 Ac 元件和几个细菌和植物标记基因，我们设计了一种多功能系统，用于识别转座子已切除并重新插入基因组其他位置的植物。转座子的设计目的是通过包含缺乏启动子或具有最小启动子序列的β-葡萄糖醛酸酶（GUS）基因来促进启动子下游和增强子附近插入的识别。该系统允许转座子和转座酶源稳定地维持在不同的植物中或同一植物中。发生转座的植物可以通过 GUS 基因的频繁体细胞激活来识别。除草剂氯磺隆用作选择剂来鉴定转座子已从其耐氯磺隆乙酰乳酸合酶基因内的原始插入位点切除的后代植物。额外的选择标记允许鉴定含有转座元件但缺乏转座酶的植物。在这里，我们描述了该系统的初步特征，并证明了其在识别具有转位元件的植物方面的可靠性和效率。
The maize transposable element Activator (Ac) has been shown to be active in a number of dicots, including Arabidopsis thaliana, whose small genome and short generation time have favored its wide adoption as a model organism for molecular genetic approaches to plant physiology and development. Using the Ac element and several bacterial and plant marker genes, we have devised a versatile system for identifying plants in which a transposon has excised and reinserted elsewhere in the genome. The transposons have been designed to facilitate the identification of insertions downstream of promoters and in the vicinity of enhancers by the inclusion of a beta-glucuronidase (GUS) gene either lacking a promoter or having a minimal promoter sequence. The system permits the transposon and the source of transposase to be maintained either stably in separate plants or in the same plant. Plants in which transposition is occurring can be identified by the frequent somatic activation of the GUS gene. The herbicide chlorsulfuron is used as a selective agent to identify progeny plants in which the transposon has excised from its original insertion site within a chlorsulfuron-resistant acetolactate synthase gene. Additional selectable markers permit the identification of plants containing a transposed element, but lacking transposase. Here we describe our initial characterization of the system and demonstrate its reliability and efficiency in identifying plants with transposed elements.
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影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
转基因拟南芥中 tms2 基因的光敏色素控制：选择信号转导途径突变体的策略。
DOI：
10.1105/tpc.3.6.573
发表时间：
1991
期刊：
The Plant cell
影响因子：
--
作者：
Karlin-Neumann,GA;Brusslan,JA;Tobin,EM
通讯作者：
Tobin,EM
Ac 转座和 DNA 复制的分子分析。
影响因子：
3.3
作者：
Chen,J;Greenblatt,IM;Dellaporta,SL
通讯作者：
Dellaporta,SL
影响因子：
3.3
作者：
P. Athma;Erich Grotewold;Thomas Peterson
通讯作者：
P. Athma;Erich Grotewold;Thomas Peterson