Measurement of non-steady-state free fatty acid turnover.
Measurement of non-steady-state free fatty acid turnover.
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非稳态游离脂肪酸周转率的测量。
DOI：
10.1152/ajpendo.1990.258.1.e103
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Miles,JM
中科院分区：
文献类型：
--
作者：
Jensen,MD;Heiling,V;Miles,JM
用于测量游离脂肪酸出现率（Ra）变化的非稳态方程的准确性尚不清楚。在本研究中，6只有意识的杂种狗的内源性脂肪分解（追踪到[14C]-亚油酸）被药理学抑制。然后使用计算机响应输液泵以恒定和线性梯度两种方式静脉输送油酸乳剂。采用不同有效分布体积(V)的非稳态方程和稳态方程测量油酸盐Ra [（14C]油酸盐）。内源性脂肪分解在实验过程中没有变化。当油酸Ra以线性梯度方式增加时，只有大V （150 ml/kg）的非稳态方程会导致错误值（高估9%，P < 0.05）。相反，当油酸Ra以类似的方式下降时，稳态和标准非稳态方程（V =血浆体积= 50 ml/kg）高估了总油酸Ra（18%和7%，P分别小于0.001和0.05）。总的来说，有效V为90 ml/kg（1.8倍血浆体积）的非稳态方程允许最准确地估计油酸Ra。
The accuracy of non-steady-state equations for measuring changes in free fatty acid rate of appearance (Ra) is unknown. In the present study, endogenous lipolysis (traced with [14C]-linoleate) was pharmacologically suppressed in six conscious mongrel dogs. A computer-responsive infusion pump was then used to deliver an intravenous oleic acid emulsion in both constant and linear gradient infusion modes. Both non-steady-state equations with various effective volumes of distribution (V) and steady-state equations were used to measure oleate Ra [( 14C]oleate). Endogenous lipolysis did not change during the experiment. When oleate Ra increased in a linear gradient fashion, only non-steady-state equations with a large (150 ml/kg) V resulted in erroneous values (9% overestimate, P less than 0.05). In contrast, when oleate Ra decreased in a similar fashion, steady-state and standard non-steady-state equations (V = plasma volume = 50 ml/kg) overestimated total oleate Ra (18 and 7%, P less than 0.001 and P less than 0.05, respectively). Overall, non-steady-state equations with an effective V of 90 ml/kg (1.8 x plasma volume) allowed the most accurate estimates of oleate Ra.
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影响因子：
4.1
作者：
ALLSOP, JR;WOLFE, RR;BURKE, JF
通讯作者：
BURKE, JF
影响因子：
15.9
作者：
EATON, RP;BERMAN, M;STEINBERG, D
通讯作者：
STEINBERG, D
DOI：
10.1152/ajplegacy.1960.199.5.797
发表时间：
1960
期刊：
The American journal of physiology
影响因子：
--
作者：
S. Deavers;E. L. Smith;R. Huggins
通讯作者：
R. Huggins
影响因子：
15.9
作者：
JENSEN, MD;HAYMOND, MW;MILES, JM
通讯作者：
MILES, JM